ArticleVeterinary research communications2025
Evaluating DAPI stain to assess sperm membrane integrity by flow cytometry in livestock species.
Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Looking at the fraction with Annexin V⁺ and propidium iodide⁺: insights into cell death types from preclinical studies in solid and haematological cancers.Apoptosis : an international journal on programmed cell death · 2026Review
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9 authors.
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Abstract
Sperm viability is one of the key in vitro parameters of sperm quality closely related to fertility. Typically, sperm viability is assessed by flow cytometry (FC) with propidium iodide (PI) as the fluorescent stain. However, as PI emits in the red spectrum, it can overlap with other commonly used fluorochromes. Utilizing a blue viability stain, such as DAPI, for semen evaluation would free the red channel, enabling the use of additional fluorochromes and expanding analytical possibilities. This study aimed to evaluate the efficiency of DAPI as a viability stain for FC in three livestock species (goat, rabbit, and cattle), for which three experiments were conducted. First, the effect of incubation time on sperm viability rates was examined with DAPI and PI. Results indicated that incubation time did not affect the sperm permeability when DAPI and PI were employed. Second, the efficiency of DAPI as a viability indicator on different livestock species was studied. Viability rates measured with DAPI in goat, rabbit, and bull sperm samples showed significant correlation with PI measurements, showing values of 0.87, 0.86, and 0.61, respectively (P < 0.01). Third, the effect of intrasample variation on viability rate was analyzed. When bovine sperm samples were stained simultaneously with DAPI and PI, the correlation between the two protocols was 0.83. In conclusion, DAPI is an alternative to PI for assessing sperm viability in livestock species, whilst also highlighting the importance of possible inter-species differences.
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