Evidence map›Paper›PMID 41115935›Full record

ArticleDiabetologia2026

Oxidative stress-regulatory role of miR-10b-5p in the diabetic human cornea revealed through integrated multi-omics analysis.

Daxian Zha, Joshua Gamez, Shaghaiegh M Ebrahimi, Yizhou Wang, Nagendra Verma, Adam J Poe, Seok White, Ruchi Shah, Andrei A Kramerov, Onkar B Sawant and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetologia, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Circulating miR-10b-5p as a candidate biomarker of atrial fibrillation recurrence after catheter ablation: a two-phase translational study.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2026
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Daxian ZhaBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Joshua GamezBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Shaghaiegh M EbrahimiBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Yizhou WangDepartment of Computational Biomedicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Nagendra VermaBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Adam J PoeBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Seok WhiteBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Ruchi ShahBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Andrei A KramerovBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Onkar B SawantCenter for Vision and Eye Banking Research, Eversight, Cleveland, OH, USA.
Chintda SantiskulvongGenomics Core, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Aleksandr B StotlandAdvanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Zhiping P WangDepartment of Computational Biomedicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Jennifer E Van EykAdvanced Clinical Biosystems Research Institute, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Alexander V LjubimovBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
Mehrnoosh SaghizadehBiomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Mehrnoosh.ghiam@cshs.org.ORCID 0000-0002-4956-569X

Funding

Mechanisms of Epithelial Alterations in Diabetic CorneaR01EY013431 · CEDARS-SINAI MEDICAL CENTER · 2001 to 2025
$2.0M
The Role of MicroRNAs in Normal and Diseased Corneal Epithelial HomeostasisR01EY025377 · CEDARS-SINAI MEDICAL CENTER · 2025 to 2025
$472k
NEI NIH HHS R01 EY013431NEI NIH HHS R01 EY025377NEI NIH HHS R01 EY029829NEI NIH HHS R01 EY031377NIH/NEI R01EY013431 (AVL)NIH/NEI R01EY025377 (MS)NIH/NEI R01EY029829 (MS)NIH/NEI R01EY031377 (AVL)the California Institute for Regenerative Medicine CIRM EDUC4-12751
6 · The paper itself

Abstract

aims/hypothesisProminent features of diabetic corneal disease are oxidative stress, neuropathy and epitheliopathy including delayed wound healing and dysfunction of limbal epithelial stem cells. We hypothesised that regulatory miRNAs altered in the diabetic cornea, such as miR-10b-5p, may be responsible for these abnormalities. We aimed to understand the molecular impact of miR-10b-5p increase in human diabetic vs non-diabetic limbal epithelial cells (LECs) enriched in limbal epithelial stem cells by identifying its target genes and proteins and testing it as a potential therapy for inhibiting oxidative stress in diabetic corneas.

methodsLECs were isolated from diabetic and non-diabetic human autopsy corneas. Telomerase-immortalised human corneal epithelial cells (HCECs), primary LECs and ex vivo organ-cultured corneas were transfected with 50 nmol/l hsa-miR-10b-5p mimic or miRNA inhibitor or siRNA against GCLM (glutamate-cysteine ligase modifier subunit) along with their respective controls using Lipofectamine RNAiMAX. Total RNA was extracted for transcriptomic analysis. Proteins were extracted, digested and quantified using LC-MS/MS proteomics. Oxidative stress was induced using 200 µmol/l hydrogen peroxide (H

resultsIntegrative proteomic and genomic analysis of miR-10b- vs miRNA mimic control-transfected primary LECs identified GCLM and LANCL1 as key miR-10b-5p targets (false discovery rate p<0.05), validated by western blot and immunostaining. miR-10b- and siRNA-GCLM-transfected LECs 3 h after H CONCLUSIONS/

interpretationOur findings suggest that diabetes-overexpressed miR-10b disrupts redox balance by targeting GCLM and LANCL1, which potentially leads to increased oxidative stress and cellular vulnerability in diabetic corneas. Inhibiting miR-10b-5p restored antioxidant defences, suggesting a potential therapeutic strategy to mitigate oxidative stress and normalise corneal health in individuals with diabetes and preserve vision.

Indexed as

CorneaMicroRNAsOxidative StressCells, CulturedGlutamate-Cysteine LigaseHumansHydrogen PeroxideMaleMiddle AgedMultiomicsGCLM protein, humanGlutamate-Cysteine LigaseHydrogen PeroxideMicroRNAsMIRN10 microRNA, humanCorneal limbal stem cellsDiabetic corneaGCLMGlutamate-cysteine ligase (GCL)Glutathione (GSH)LANCL1miR-10b-5pmiRNAOxidative stressProteomicsTranscriptomics

Identifiers

PMID41115935
PMCPMC12686106

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.