Evidence map›Paper›PMID 41120393›Full record

ArticleScientific reports2025

Plasmid-mediated quinolone resistance among extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from hospitalized patients, hospital environment and wastewaters in Cameroon.

Patrice Landry Koudoum, Raspail Carrel Founou, Luria Leslie Founou, Megane Daina Foueyem, Giresse Wilfried Guemkam, Richard Deuguen, Gisele Ateba Nke, Hortense Gonsu, Simeon Pierre Choukem

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Patrice Landry KoudoumDepartment of Microbiology- Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
Raspail Carrel FounouDepartment of Microbiology- Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon. czangue@yahoo.fr.
Luria Leslie FounouAntimicrobial Research Unit, School of Health Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, 4000, South Africa.
Megane Daina FoueyemDepartment of Microbiology- Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
Giresse Wilfried GuemkamDepartment of Microbiology- Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
Richard DeuguenDepartment of Microbiology- Haematology and Immunology, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
Gisele Ateba NkeYaoundé Central Hospital, Yaoundé, Cameroon.
Hortense GonsuFaculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.
Simeon Pierre ChoukemDepartment of Internal Medicine and Specialties, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) and Klebsiella pneumoniae (ESBL-Kp) are among the leading causes of hospital-acquired infections globally. Fluoroquinolone-resistant ESBL-Ec/Kp infections have limited therapeutic options. This study investigated the prevalence and antibiotic resistance patterns of ESBL-Ec/Kp isolated from hospitalized patients, hospital environments, and wastewaters in Yaoundé, Cameroon. It further characterized ESBL genes and clonal relatedness in all ESBL-Ec/Kp isolates and assessed plasmid-mediated quinolone resistance (PMQR) genes specifically in ciprofloxacin-resistant isolates. A cross-sectional study was conducted from February to June 2024 in two healthcare facilities in Yaoundé, Cameroon. Clinical, inanimate surfaces, and wastewater samples were collected. Bacteria identification was done using the API20E kit. The ESBL phenotype was detected using the double-disk synergy test and CHROMagar™ ESBL. Antimicrobial susceptibility testing was performed using the disc diffusion method. Genes conferring resistance to β-lactams and fluoroquinolones were detected using polymerase chain reaction (PCR). Clonal relatedness was assessed using enterobacterial repetitive intergenic consensus (ERIC)-PCR. The overall ESBL prevalence across all sources was 16% (103/652). This ESBL prevalence was 10% (49/495) in hospitalized patients, 27% (38/141) in the hospital environment, and 100% (16/16) in hospital wastewaters. Nearly all (99.5%) ESBL-Ec and ESBL-Kp were multidrug-resistant. The bla

Indexed as

beta-LactamasesDrug Resistance, BacterialEscherichia coliKlebsiella pneumoniaePlasmidsQuinolonesWastewaterAnti-Bacterial AgentsCameroonCross InfectionCross-Sectional StudiesDrug Resistance, Multiple, BacterialEscherichia coli InfectionsFemaleHospitalsHumansAnti-Bacterial Agentsbeta-LactamasesQuinolonesWastewaterEnterobacteralesExtended-Spectrum β-LactamaseHospital-acquired infectionHospital environmentOne healthPlasmid-Mediated quinolone resistance

Identifiers

PMID41120393
PMCPMC12540776

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.