ArticleFrontiers in veterinary science2025
Isolation and genetic characterization of a novel recombinant HP-PRRSV strain in Jiangxi Province, China.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Integrated Single-Cell and TCR Profiling Reveals Protection-Associated CD8Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The updated one-step multiplex RT-qPCR method for PRRSV classical strains, highly pathogenic strains and NADC30-like strains.Frontiers in microbiology · 2026Article
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Authors and funding
7 authors.
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Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen responsible for significant economic losses in the global pig industry, primarily causing reproductive failure in sows and respiratory diseases in piglets. In this study, we isolated a novel PRRSV strain, designated NC2023, from clinical samples collected in 2023 from a pig farm in Jiangxi Province, China. The virus was successfully propagated in Marc-145 cells and demonstrated consistent infectivity in iPAM cells. Comprehensive characterization of NC2023 was performed using RT-qPCR, Western blotting, IFA, electron microscopy, plaque purification, and whole-genome sequencing. Phylogenetic and homology analyses of the complete genome revealed that NC2023 belongs to lineage 8 of the PRRSV-2 subtype and forms an independent branch in the evolutionary tree. The genomic length of NC2023 is 15,321 nucleotides, and recombination analysis indicated it is a recombinant strain with specific genomic regions derived from strains JXA1, JXA1-R, and HUN4. Notably, NC2023 exhibits 15 unique amino acid mutations compared to other recombinant strains, along with a 395-amino-acid frameshift mutation in Nsp2. This study reports the isolation and characterization of an HP-PRRSV recombinant PRRSV-2 strain using Marc-145 and iPAM cells, providing key insights into the genetic variation and evolutionary dynamics of PRRSV. These findings contribute to a deeper understanding of PRRSV molecular epidemiology and have important implications for the development of effective control strategies and vaccines.
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