Evidence mapPaperPMID 41125618Full record

ArticleScientific reports2025

Integrated transcriptomic analysis identifies lysosomal autophagy-related genes in sarcopenia.

Ye Zhou, Yue Qian, Xin Yuan, Qingqing Zhu

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Ye ZhouDepartment of Geriatrics, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou, 310006, Zhejiang, China.
Yue QianHealth and Wellness Center, WuYunShan Hospital of Hangzhou (Health Promotion Research Institute of Hangzhou), Hangzhou, 310013, Zhejiang, China. qianyue@hzswysyy.com.
Xin YuanSchool of Engineering, Westlake University, Hangzhou, 310030, Zhejiang, China.
Qingqing ZhuDepartment of Radiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 311500, Zhejiang, China.

Funding

Hangzhou Health Science and Technology Plan Projects for 2025 A20251673
6 · The paper itself

Abstract

Sarcopenia (SARC) presents considerable challenges to the quality of life for the elderly and exerts a significant economic strain on healthcare systems. Contemporary therapeutic methods, such as physical activity, dietary supplementation, and medication, frequently demonstrate restricted effectiveness and significant individual variation. This study seeks to identify new therapeutic targets through the analysis of lysosomal autophagy-related differentially expressed genes (LARDEGs) associated with SARC. Differentially expressed genes(DEGs) related to SARC were identified through an extensive analysis of microarray datasets (GSE8479 and GSE1428) obtained from the Gene Expression Omnibus. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction (PPI) network analysis, and Gene Set Enrichment Analysis were used to assess the biological functions, molecular pathways, autophagy-related molecular markers, and immune microenvironment linked to SARC DEGs. Our findings identified 12 LARDEGs, including BHLHE41, HLTF, UBE2D1, UBE2D2, NPC1, NDRG1, SLC22A18, CDKN1A, CALCOCO2, SCARB2, LGALS1, and RPS27A, that were significantly correlated with energy metabolism and mitochondrial function. We constructed a PPI network that identified six crucial hub genes: BHLHE41, UBE2D1, UBE2D2, CDKN1A, SCARB2, and RPS27A. This network provides significant insights into their functional roles and their potential as therapeutic targets. Additionally, an examination of immune infiltration demonstrated significant differences in the quantities of resting natural killer (NK) cells and M2 macrophages between SARC samples and control samples. CDKN1A displayed a positive correlation with M2 macrophages and an inverse relationship with resting NK cells. The results show how important the immune microenvironment is to the spread of SARC, suggesting promising pathways for the creation of immunotherapeutic strategies. Our research elucidates the molecular pathways implicated in SARC and establishes a foundation for future therapeutic approaches. However, additional validation is crucial to translating these findings into viable clinical applications.

Indexed as

AutophagyGene Expression ProfilingLysosomesSarcopeniaTranscriptomeGene OntologyGene Regulatory NetworksHumansProtein Interaction MapsDifferentially expressed genesImmune infiltrationIntegrated transcriptomic analysisLysosomal autophagySarcopenia

Identifiers

PMID41125618
PMCPMC12546856

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.