Evidence map›Paper›PMID 41126028›Full record

ArticleBMC genomics2025

Genome-wide identification of the WIP family in foxtail millet (Setaria italica) and functional analysis of SiWIP3 in inhibiting growth in transgenic Arabidopsis thaliana.

Xiling Chang, Tianqi Song, Jiandong Ren, Xiaoxing Wang, Jianfei Zhou, Yaning Bu, Xiaohong Chen, Yuxin Wang, Siyi Li, Xinpeng Lei and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiling ChangCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Tianqi SongCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Jiandong RenCollege of Biological Sciences and Technology, Yili Normal University, Yili, Xinjiang, 830500, China.
Xiaoxing WangCollege of Biological Sciences and Technology, Yili Normal University, Yili, Xinjiang, 830500, China.
Jianfei ZhouCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Yaning BuCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Xiaohong ChenCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Yuxin WangCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Siyi LiCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Xinpeng LeiCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China.
Jishan XiangCollege of Biological Sciences and Technology, Yili Normal University, Yili, Xinjiang, 830500, China. xiangjsh@126.com.
Xiaoke ZhangCollege of Agronomy, Northwest A & F University, Yangling, Xianyang, Shaanxi, 712100, China. zhangxiaoke66@126.com.

Funding

Natural Science Foundation of Inner Mongolia Autonomous Region 2023JQ10Research Program of Science and Technology at Universities of Inner Mongolia Autonomous Region NJZZ22132Xinjiang Tianchi Talents Introduction Program 2024TCYCCXLJ01
6 · The paper itself

Abstract

The wound-induced protein (WIP) is a member of the A1d subfamily of C2H2 zinc finger proteins and plays a crucial role in plant growth and development. Foxtail millet (Setaria italica) serves as a model organism for research on C4 crops. To date, the WIP family has been identified in several plant species, including Arabidopsis thaliana and tomato (Solanum lycopersicum L.), but it has not yet been reported in foxtail millet. In this study, we conducted bioinformatics analysis to identify five SiWIP genes in the whole genome of foxtail millet, and further examined their chromosomal distribution, gene structure, cis-elements, and conserved protein motifs. The analysis of tissue expression patterns of SiWIP3, SiWIP4 and SiWIP5 members, as well as their response to exogenous hormone treatments, indicates that SiWIPs demonstrate tissue specificity and exhibit distinct reactions to exogenous hormones. Notably, SiWIP3 displays a certain level of sensitivity to exogenous hormones and shows the highest expression in roots. Further research on SiWIP3 has revealed its nuclear localization, and the overexpression of SiWIP3 in Arabidopsis results in a reduction in leaf size, slower plant growth, and suppressed flowering. However, the inhibition was relieved by the exogenous application of gibberellin (GA3), leading to leaf expansion and promotion of flowering. RT-qPCR results revealed alterations in the expression levels of GA pathway related genes in Arabidopsis overexpressed lines, both before and after GA3 treatment. In addition, the dual luciferase assay demonstrated that SiKNOX1 or SiBPC6 significantly suppressed the expression of the SiWIP3. Protein interactions between SiWIP3 and SiTCP19 were confirmed through yeast two-hybrid and luciferase complementation imaging assay experiments. SiWIP3 may play a crucial role in GA pathway, thereby regulating the growth and development in foxtail millet. This research provides a fundamental basis for further elucidating the mechanism of WIP protein in plant growth and development.

Indexed as

ArabidopsisPlant ProteinsSetaria PlantGene Expression Regulation, PlantGenome, PlantMultigene FamilyPhylogenyPlants, Genetically ModifiedPlant ProteinsFoxtail milletGrowth and developmentThe C2H2 gene familyWound-induced protein (WIP)

Identifiers

PMID41126028
PMCPMC12542068

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.