ArticleParasites & vectors2025
Impact of Asaia bacteria on Leishmania major development in sand flies: implications for vector control strategies.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Biopesticides as an eco-friendly approach to mosquitoes and their role in reducing transmission of vector-borne disease: updates and prospects.Pest management science · 2026Review
- New Advancements in the Field of Leishmaniasis.Microorganisms · 2026Article
- Confronting the known unknown: historical lessons and future strategies for Disease X.Frontiers in public health · 2026Review
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7 authors.
Funding
Abstract
backgroundAsaia spp., bacteria originally isolated from tropical plants, have also been identified in various insect species, including blood-feeding ones. Their ability to colonize different host tissues and transmit vertically between generations makes these bacteria good candidates for paratransgenesis. However, most existing data derived from studies on mosquitoes and other important vectors, such as phlebotomine sand flies (Diptera: Psychodidae), remain understudied. In this study, we investigated the ability of wild-type Asaia siamensis, Asaia krungthepensis, and a genetically modified strain of Asaia expressing the Wolbachia surface protein (Asaia
methodsPhlebotomus duboscqi females were provided with Asaia via a sugar meal. Bacterial presence and vertical transmission were assessed using both cultivation and polymerase chain reaction (PCR). In superinfection experiments, females were first offered sugar containing Asaia, followed by a blood meal infected with Le. major. The outcomes of superinfection were assessed by cultivation, PCR, and microscopically. Statistical analyses were performed using Fisher's exact or Chi-squared tests.
resultsAll tested Asaia strains colonized the gut of Ph. duboscqi. Both A. siamensis and A. krungthepensis were vertically transmitted to the progeny via egg smearing. These bacteria did not affect the infection rate and intensity of Le. major infection on days 2 and 5 post blood meal (pbm). However, by day 8 pbm, both species significantly reduced Le. major infection intensity. Moreover, A. krungthepensis significantly increased the proportion of metacyclic forms. Interestingly Asaia
conclusionsWe demonstrated for the first time that A. siamensis and A. krungthepensis can infect Ph. duboscqi and be vertically transmitted to the next generation via egg smearing. These bacteria affect the late phase of Le. major infection, which could have important epidemiological consequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.