Evidence map›Paper›PMID 41126997›Full record

ArticlePNAS nexus2025

Manipulation of the nucleoscaffold potentiates cellular reprogramming kinetics.

Benjamin A Yang, Camila Vesga-Castro, André Monteiro da Rocha, Isabel Newton, Anna Shcherbina, Sing-Wan Wong, Paula M Fraczek, Jacqueline A Larouche, Harrison Hiraki, Brendon M Baker and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Trained immunity in respiratory diseases: Mechanisms of action and intervention strategies.Chinese medical journal pulmonary and critical care medicine · 2026
    Review
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Benjamin A YangDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0001-7412-6261
Camila Vesga-CastroDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
André Monteiro da RochaDepartment of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0003-3324-3773
Isabel NewtonDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Anna ShcherbinaDepartment of Biomedical Informatics, Stanford University, Palo Alto, CA 94305, USA.ORCID https://orcid.org/0000-0002-5080-5494
Sing-Wan WongDepartment of Pharmacology and Biomedical Engineering, University of Illinois at Chicago, Chicago, IL 60612, USA.
Paula M FraczekDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0002-9897-0720
Jacqueline A LaroucheDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0001-9380-3547
Harrison HirakiDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0002-6199-0744
Brendon M BakerDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Jae-Won ShinDepartment of Pharmacology and Biomedical Engineering, University of Illinois at Chicago, Chicago, IL 60612, USA.
Shuichi TakayamaWallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.ORCID https://orcid.org/0000-0002-4385-9080
M D ThoulessDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Carlos A AguilarDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID https://orcid.org/0000-0003-3830-0634

Funding

Research Education CoreP30AG024824 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lona Mody, RAYMOND L YUNG · 2004 to 2026
$29.2M
Structure, Composition, & Histology Core - Core BP30AR069620 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KARL J JEPSEN · 2016 to 2026
$8.4M
Microfluidics in Biomedical Sciences Training ProgramT32EB005582 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FAN, XUDONG · 2005 to 2020
$3.9M
Super-resolution visualization of histones and histone modifications on individual chromatin fibersR01GM123517 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI LIU, YIFAN, TAKAYAMA, SHUICHI · 2017 to 2020
$1.6M
NIAMS NIH HHS P30 AR069620NIA NIH HHS P30 AG024824NIBIB NIH HHS T32 EB005582NIGMS NIH HHS R01 GM123517
6 · The paper itself

Abstract

Somatic cell fate is an outcome set by the activities of specific transcription factors and the chromatin landscape and is maintained by gene silencing of alternate cell fates through physical interactions with the nuclear scaffold. Here, we evaluate the role of the nuclear scaffold as a guardian of cell fate in human fibroblasts by comparing the effects of transient loss (knockdown) and mutation (progeria) of functional Lamin A/C, a core component of the nuclear scaffold. We observed that Lamin A/C deficiency or mutation disrupts nuclear morphology, and the mechanical properties of the nucleus when measured by a microfluidic cellular squeezing device. We also show that transient loss of Lamin A/C promotes opening of previously silenced heterochromatin domains and increases access to DNA in lamina-associated domains. These alterations in Lamin A/C resulted in acceleration of the kinetics of cellular reprogramming to pluripotency, while genetic mutation of Lamin A/C into progerin was found to induce a senescent phenotype that inhibits the induction of reprogramming genes. Our results highlight the physical role of the nuclear scaffold in safeguarding cellular fate.

Identifiers

PMID41126997
PMCPMC12538391

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.