Evidence mapPaperPMID 41129337Full record

ArticleShock (Augusta, Ga.)2026

Identification and Validation of Key Genes Related to Arginine Methylation Modification in Sepsis Using Transcriptome Combined with Mendelian Randomization Analysis.

Peng Huang, Meifeng Wang, Weihong Hong, Jinyuan Kang, Yuyang Li, Ying Li, Xiao Lin

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Article in Shock (Augusta, Ga.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Peng HuangDepartment of Intensive Care Unit, The First Affliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Meifeng WangDepartment of Pathology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China.
Weihong HongDepartment of Intensive Care Unit, The First Affliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Jinyuan KangDepartment of Intensive Care Unit, Yongchun County Hospital, Quzhou, Fujian, China.
Yuyang LiDepartment of Intensive Care Unit, Zhouning County Hospital, Ningde, Fujian, China.
Ying LiDepartment of Intensive Care Unit, The First Affliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.
Xiao LinDepartment of Intensive Care Unit, The First Affliated Hospital, Fujian Medical University, Fuzhou, Fujian, China.

Funding

Startup Fund for Scientific Research of Fujian Medical University 2022QH1061
6 · The paper itself

Abstract

backgroundPatients with sepsis often exhibit a decrease in lymphatic numbers, which can be facilitated by protein arginine methyltransferase (PRMT). However, it is unclear how PRMT contributes to lymphopenia in sepsis.

methodsThis study employed the sepsis-related datasets (GSE65682 and GSE134347) and nine PRMT genes. First, we intersected the differentially expressed genes with the weighted gene co-expression network analysis module genes to identify differentially expressed PRMT-related genes. Thereafter, candidate key genes were obtained after Mendelian randomization analysis and machine learning screening. Eventually, we subjected key genes identified by expression analysis and receiver operating characteristic curves to gene set enrichment analysis, immune infiltration analysis, immune checkpoint analysis, molecular docking, regulatory networks construction, and nomogram development.

resultsWe first intersected 4,246 differentially expressed genes with 1,884 PRMT scoring module genes to obtain 969 differentially expressed PRMT-related genes. Further Mendelian randomization analysis and machine learning jointly identified five candidate genes ( CRLF3 , ELAC2 , PBX2 , MCTP2 , and EMB ). Among these, ELAC2 , PBX2 , MCTP2 , and EMB demonstrated consistent expression trends, with the area under the curve values of the receiver operating characteristic curve exceeding 0.7 in GSE65682 and GSE134347. Therefore, they were defined as key PRMT-related genes. The gene set enrichment analysis showed enrichment in cytoplasmic translation ( ELAC2 , MCTP2 ), noncoding RNA metabolism ( EMB ), and metabolic processes ( PBX2 ). The immune infiltration analysis revealed a significant correlation between PBX2 and neutrophils, as well as between ELAC2 / MCTP2 / EMB with activated natural killer cells, CD8+ T cells.

conclusionIn this study, ELAC2 , PBX2 , MCTP2 , and EMB were identified as key genes related to PRMT for sepsis, which provided a theoretical basis for the study of sepsis.

Indexed as

ArginineMendelian Randomization AnalysisProtein-Arginine N-MethyltransferasesSepsisTranscriptomeGene Expression ProfilingHumansMethylationArginineProtein-Arginine N-MethyltransferasesKey genesMendelian randomization analysisprotein arginine methyltransferasesepsistranscriptome

Identifiers

PMID41129337
PMCPMC13384385

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.