Evidence map›Paper›PMID 41134133›Full record

ArticleeLife2025

Single-cell transcriptome sequencing for opening the blood-brain barrier through specific mode electroacupuncture stimulation.

Congcong Ma, Zhaoxing Jia, Tianxiang Jiang, Qian Cai, Jinding Yang, Lin Gan, Kecheng Qian, Zixin Pan, Qinyu Ye, Mengyuan Dai and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Congcong Ma *The Third Affiliated Hospital of Zhejiang Chinese Medical University, Moganshan, China.
Zhaoxing Jia *The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Tianxiang Jiang *The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Qian Cai *The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Jinding Yang *The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Lin GanThe Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Kecheng QianThe Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Zixin PanThe Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Qinyu YeThe Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.
Mengyuan DaiDepartment of Rehabilitation, Lishui Central Hospital, Lishui, China.
Xianming LinThe Third Affiliated Hospital of Zhejiang Chinese Medical University, Moganshan, China.ORCID https://orcid.org/0000-0002-2729-8324

Funding

National Natural Science Foundation of China 82174502National Natural Science Foundation of China 82474626Scientific Research Project of the Affiliated Hospital of Zhejiang Chinese Medical University 2022FSYYZZ10Scientific Research Project of the Affiliated Hospital of Zhejiang Chinese Medical University 2023FSYYZQ11Special Project of Modernization of Traditional Chinese Medicine in Zhejiang Province 2022ZX009Zhejiang Provincial Natural Science Foundation LQN25H270008
6 · The paper itself

Abstract

The blood-brain barrier (BBB) interferes with the treatment of central nervous system disorders owing to the complexity of its structure and restrictive function. Thus, it is challenging to develop central nervous system drug delivery strategies. Specific mode electroacupuncture (EA) stimulation can effectively open the BBB in rats. Here, we used single-cell RNA sequencing (scRNA-seq) to comprehensively map the cell population in the Sprague-Dawley rat cerebral cortex. We identified 23 cell subsets and eight types of cells in the brain by cell annotation. scRNA-seq revealed transcriptional changes in the cerebral cortex under EA. Our findings offer valuable insights into the molecular and cellular modifications in the brain resulting from EA intervention and serve as a resource for drug delivery across healthy and diseased states. Innovative approaches to enhance BBB opening will lead to more effective therapeutic plans and enhanced drug delivery.

Indexed as

Blood-Brain BarrierElectroacupunctureSingle-Cell AnalysisTranscriptomeAnimalsCerebral CortexGene Expression ProfilingMaleRatsRats, Sprague-DawleySequence Analysis, RNAbioinformatics analysisblood-brain barriercentral nervous systemdrug deliverymouseneurosciencesingle-cell RNA sequencingtranscriptional change

Identifiers

PMID41134133
PMCPMC12552013

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.