Evidence map›Paper›PMID 41136476›Full record

ArticleScientific reports2025

Repulsive guidance molecule A in the resolution of inflammation from ischemic stroke-associated pneumonia in mice.

Jiaju Zhong, Taoying Xiong, Xiaoqin Luo, Lingcong Li, Wenwen Li, Yue Ma, Xiaohui Wu, Jing Wang, Rongrong Zhang, Xinyue Qin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jiaju Zhong *Department of Rehabilitation Medicine, Chongqing Key Laboratory of Cerebrovascular Disease Research, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, China.
Taoying Xiong *Department of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Xiaoqin LuoDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Lingcong LiDepartment of Rehabilitation Medicine, Chongqing Key Laboratory of Cerebrovascular Disease Research, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, China.
Wenwen LiSchool of Basic Medical Sciences, Department of Pathology, Chongqing Medical University, Chongqing, 400016, China.
Yue MaDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Xiaohui WuDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Jing WangDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Rongrong ZhangDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. zhangrongrong@cqmu.edu.cn.
Xinyue QinDepartment of Neurology, Chongqing Key Laboratory of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. xyqxinyueqin@163.com.

Funding

Natural Science Foundation of Chongqing, China CSTB2022NSCQ-MSX0072the Fund of Mountaintop Plan Project in the First Affiliated Hospital of Chongqing Medical University cyyy-xkdfjh-jcyj-202309the National Natural Science Foundation of China 82101375 and 82071338the Research Project of Yongchuan Hospital Chongqing Medical University YJSJ202136the Science and Technology Research Program of Chongqing Municipal Education Commission KJZD-K202300409the Young Outstanding Foundation of The First Affiliated Hospital of Chongqing Medical University ZYRC2020-02
6 · The paper itself

Abstract

Stroke is an important global health concern. The most prevalent infectious complication after stroke is stroke-associated pneumonia (SAP), which severely impairs recovery of neurological function and has an adverse impact on the prognosis. However, the pathogenesis of SAP is largely unknown. We established a model of SAP by middle cerebral artery occlusion/reperfusion (MCAO/R) and identified spontaneous pneumonia in mice with MCAO/R by staining the lung tissue with hematoxylin-eosin and measuring the bacterial load in the lungs and expression levels of inflammatory factors in plasma. We also confirmed expression of repulsive guidance molecule A (RGMa) in splenic mononuclear cells (SpMNCs) following MCAO/R surgery. Recombinant mouse RGMa protein, propranolol, and the glucocorticoid receptor blocker RU486 were administered to investigate the effects of RGMa on SAP and elucidate the underlying mechanism. Marked spontaneous pneumonia was observed in the mice that had undergone MCAO/R. RGMa expression in SpMNCs from these mice was significantly reduced at 24 and 72 h following MCAO/R surgery. Exogenous administration of recombinant mouse RGMa markedly attenuated spontaneous pneumonia in MCAO/R mice. After administration of propranolol, there was a significant increase in the expression level of RGMa in SpMNCs and amelioration of spontaneous pneumonia in these mice. Administration of RU486 did not significantly change the RGMa expression level or ameliorate spontaneous pneumonia. RGMa attenuated spontaneous pneumonia in mice that had undergone MCAO/R. The underlying mechanism may involve regulation of sympathetic excitation. RGMa may play a key role in SAP.

Indexed as

GPI-Linked ProteinsInflammationIschemic StrokeNerve Tissue ProteinsPneumoniaAnimalsDisease Models, AnimalInfarction, Middle Cerebral ArteryLungMaleMiceMice, Inbred C57BLMifepristonePropranololGPI-Linked ProteinsMifepristoneNerve Tissue ProteinsPropranololRgma protein, mouseIschemic strokeMiddle cerebral artery occlusion/ReperfusionPeripheral immunosuppressionRepulsive guidance molecule aStroke-associated pneumonia

Identifiers

PMID41136476
PMCPMC12552507

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.