Evidence map›Paper›PMID 41138188›Full record

ArticleSTAR protocols2025

Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry.

Matias Cardenas, Sasha Compton, Ashkon Hunt, Pradeep Chopra, Daniel R Perez, Geert-Jan Boons, Daniela S Rajao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Matias CardenasDepartment of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. Electronic address: m.cardenas@uga.edu.
Sasha ComptonDepartment of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Ashkon HuntDepartment of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Pradeep ChopraComplex Carbohydrate and Research Center, University of Georgia, Athens, GA 30602, USA.
Daniel R PerezDepartment of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Geert-Jan BoonsComplex Carbohydrate and Research Center, University of Georgia, Athens, GA 30602, USA; Department of Chemistry, University of Georgia, Athens, GA 30602, USA; Chemical Biology & Drug Discovery, Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands.
Daniela S RajaoDepartment of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA. Electronic address: daniela.rajao@uga.edu.

Funding

NIAID Centers of Excellence for Influenza Research and Response: Universal Influenza Vaccine Research Activities75N93021C00014 · NIAID · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GARCIA-SASTRE, ADOLFO · 2021 to 2025
$62.6M
Automated chemo-enzymatic synthesis of N-glycans for host-pathogen interactionsR01AI165692 · NIAID · UNIVERSITY OF GEORGIA · PI BOONS, GEERT-JAN · 2022 to 2025
$2.2M
NIAID NIH HHS 75N93021C00014NIAID NIH HHS R01 AI165692
6 · The paper itself

Abstract

Recent work has shown that major histocompatibility complex (MHC) class II is a functional receptor for some influenza A virus (FLUAV) subtypes. Here, we present a protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry. We describe steps for producing recombinant human leukocyte antigen DR isotype (HLA-DR) MHC class II using a baculovirus expression system. The HLA-DR antigen is then modified for its utilization as a biolayer interferometry (BLI) probe for kinetic experiments using whole FLUAV viruses. This protocol allows for the screening of different FLUAV subtypes and their affinity for MHC class II.

Indexed as

Histocompatibility Antigens Class IIInfluenza A virusInterferometryAnimalsBaculoviridaeHLA-DR AntigensHumansKineticsProtein BindingHistocompatibility Antigens Class IIHLA-DR AntigensBiophysicsMicrobiologyProtein expression and purification

Identifiers

PMID41138188
PMCPMC12593577

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.