Evidence map›Paper›PMID 41144196›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Cryptosporidium Diagnostic Assays: Microscopy.

Guy Robinson, Rachel M Chalmers

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Colpodellosis: IsPathogens (Basel, Switzerland) · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Guy RobinsonCryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, UK. Guy.Robinson@wales.nhs.uk.
Rachel M ChalmersCryptosporidium Reference Unit, Public Health Wales Microbiology and Health Protection, Singleton Hospital, Swansea, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Stained microscopy of fecal smears was the cornerstone of Cryptosporidium diagnosis for many years and provides a low-cost method for detecting oocysts. The development and commercialization of improved enzyme immunosorbent assays (EIAs) for coproantigen detection provided an automatable method for mass testing and rapid diagnostics when incorporated onto a cartridge format. Similarly, immunochromatographic lateral flow (ICLF) assays enable rapid diagnostics. Nevertheless, it is important that positive reactions by EIAs or ICLF assays are confirmed. Here, we describe microscopical methods using tinctorial stains for the diagnosis of acute cryptosporidiosis and using immunofluorescent reagents for diagnosis or for confirmation of EIA- or ICLF-positive reactions.

Indexed as

CryptosporidiosisCryptosporidiumMicroscopyChromatography, AffinityFecesFluorescent Antibody TechniqueHumansOocystsStaining and LabelingAuramine phenolCryptosporidiumImmunofluorescentMicroscopyOocyst detectionZiehl–Neelsen

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.