ArticleAntioxidants (Basel, Switzerland)2025
High-Power Laser Therapy Modulates Mitochondrial Function and Redox Balance Without Cytotoxicity: An In Vitro Study in BV-2 Microglial Cells.
Article in Antioxidants (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent technological advances have sparked growing interest in high-power laser devices due to their capacity for energy delivery and therapeutic potential, especially in deeper tissues. This promising approach may be comparable to photobiomodulation for modulating inflammatory and redox processes in various tissues. However, to our knowledge, this is the first study to evaluate the safety profile and redox modulation capacity of high-power laser therapy in BV-2 microglial cells.
methodsThis study investigated the cellular responses of BV-2 microglial cells exposed to three laser irradiation protocols using a high-power laser device (650/810/915/980 nm, 657 J total dose), applied at variable distances to simulate in vivo power attenuation. Cell viability, apoptosis, adenosine triphosphate(ATP) levels, mitochondrial membrane potential (MMP), reactive oxygen species (ROS), nitric oxide (NO), and intracellular calcium levels were assessed at multiple time points (5 min to 24 h).
resultsProtocol-dependent effects were observed. Protocol A promoted early increases in cell viability and ATP levels, along with decreased apoptotic markers and ROS production, suggesting a protective bioenergetic response. In contrast, Protocol C showed transient increases in oxidative stress and reduced MMP, suggesting possible mitochondrial stress. A selective increase in NO levels under Protocol A also suggests modulation of inflammatory pathways without cytotoxicity.
conclusionsHigh-power laser therapy modulates redox balance, mitochondrial function, and inflammatory mediators (e.g., NO) in a dual-phase manner in BV-2 microglial cells. These findings contribute to defining safe and effective parameters for potential musculoskeletal and neurological applications.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.