Evidence mapPaperPMID 41159692Full record

ArticleMolecular pain

Amino acid transporter SLC38A3 mediates bone cancer pain in rats via the PI3K/AKT/TRPV1 signaling pathway.

Yu-Na Cheng, Ming-Zhu Zhang, Cui-Qing Xie, Zhi-Qi Li, You-Feng Ge, Zhao-Hui Jin, Zhi-Hua Luo, Chen-Yang Zheng, Liang Chen, Dong Fang and 2 more

Abstract read
In one paragraph

Article in Molecular pain. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yu-Na ChengDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Ming-Zhu ZhangDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Cui-Qing XieDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Zhi-Qi LiDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
You-Feng GeDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Zhao-Hui JinDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Zhi-Hua LuoDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Chen-Yang ZhengDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Liang ChenDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.
Dong FangDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.ORCID 0000-0003-3538-9397
Chen-Guang NiuSchool of Stomatology, Henan University, Kaifeng, Henan, China.
Jin-Jin HeDepartment of Pharmacy, the First Affiliated Hospital of Henan University, Kaifeng, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone cancer pain (BCP), one of the most intractable symptoms in patients with cancer, remains poorly understood and lacks effective therapeutic interventions. In this study, we employed an established rat model of BCP induced by intratibial injection of MRMT-1 mammary carcinoma cells. Transcriptomic profiling of the L4-L6 dorsal root ganglia (DRGs) revealed an upregulation of the amino acid transporter SLC38A3. This finding was further confirmed by time-dependent increases in both its mRNA and protein levels. Immunofluorescence co-localization indicated that SLC38A3 was expressed in NF200-, CGRP-, and IB4-positive neurons within the L4-L6 DRGs, and its expression was upregulated in the BCP model. Concomitantly, the transient receptor potential vanilloid 1 (TRPV1) expression in BCP rat DRGs was dynamically upregulated at both the mRNA and protein levels, aligning temporally with pain hypersensitivity. Lentivirus-mediated overexpression or knockdown of SLC38A3 in the DRGs led to a corresponding upregulation or downregulation of TRPV1-expression. Activation of the PI3K/AKT signaling pathway corresponds with BCP-related pain behaviors and expression patterns of SLC38A3 and TRPV1. Bexarotene alleviates BCP in rats by suppressing the aberrant overexpression of SLC38A3, thereby blocking the PI3K/AKT signaling pathway-mediated upregulation of TRPV1. These findings indicate that SLC38A3, through its downstream PI3K/AKT-TRPV1 axis, may serve as a potential molecular mechanism for analgesia in BCP.

Indexed as

Amino Acid Transport System ABone NeoplasmsCancer PainPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTRPV Cation ChannelsAnimalsCell Line, TumorFemaleGanglia, SpinalRatsRats, Sprague-DawleyUp-RegulationAmino Acid Transport System APhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktTrpv1 protein, ratTRPV Cation ChannelsBone cancer paindorsal root ganglionPI3K/AKTSLC38A3TRPV1

Identifiers

PMID41159692
PMCPMC12644426

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.