Evidence mapPaperPMID 41163003Full record

ArticleBMC veterinary research2025

Dietary quercetin supplementation alleviates claw lesions in dairy cows by modulating the PI3K/Akt and NF-κB signaling pathways.

Changhong Zhai, Jingyi Lin, Xiaonan Yang, Jing Mu, Lei Lei, Wende Wu, Yimin Zheng, Mingxian Shi, Chunxia Ma, Hao Peng and 2 more

Abstract read
In one paragraph

Article in BMC veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Changhong ZhaiClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Jingyi LinClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Xiaonan YangNational Engineering Research Center for Southwest Endangered Medicinal Resources Development, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.
Jing MuClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Lei LeiClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Wende WuClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Yimin ZhengClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Mingxian ShiClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Chunxia MaClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China.
Hao PengGuangxi Veterinary Research Institute, Nanning, 530001, China.
Gonghe LiClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China. ligonghe@163.com.
Jiafeng DingClinical Veterinary Department, College of Animal Science and Veterinary Medicine, Guangxi University, Nanning, 530004, China. jiafengding@cau.edu.cn.

Funding

Guangxi Key Research and Development Plan AB241484045the "14th Five-Year Plan" Project of Fangchenggang Science and Technology Program Fangke 24BB108460the National Key Research and Development Program of China No. 2024YFD1301303
6 · The paper itself

Abstract

backgroundLameness, primarily caused by claw lesions, leads to serious economic losses and animal welfare problems in dairy farms worldwide. Currently, its treatment approaches include topical application of nonsteroidal anti-inflammatory drugs (NSAIDs), improvement of the feeding environment, and therapeutic hoof trimming. However, long-term administration of NSAIDs may lead to adverse effects such as gastrointestinal complications and drug residue issues in dairy products. Recently, there was an increasing interest in the application of flavonoids in livestock management. Quercetin, a naturally abundant and cost-effective flavonoid, possesses multifunctional bioactivities, including anti-inflammatory, antioxidant, and metabolic-modulating functions. The purpose of this study was to evaluate the effect of dietary quercetin addition on claw lesions in dairy cows and to elucidate the possible mechanisms involved.

resultsIn dairy cows with claw lesions, dietary supplementation with quercetin (100 mg/kg body weight) over 90 days suppressed NF-κB and PI3K/Akt signaling pathways, evidenced by inhibition of the phosphorylation of PI3K, Akt, p65, and IκBα, while downregulating their gene expression. Concomitantly, quercetin reduced the mRNA expression of inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), C-X-C motif chemokine ligand 1 (CXCL-1), C-X-C motif chemokine ligand 6 (CXCL-6), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS)) in lamellar tissues of dairy cows with claw lesions.

conclusionsIn conclusion, our findings indicate that dietary quercetin supplementation alleviates claw lesions and decreases the inflammatory response of lamellar tissues in dairy cows by modulating the PI3K/Akt and NF-κB signaling pathways. These results suggest that quercetin may serve as a natural anti-inflammatory candidate for the management of claw lesions in dairy cattle, offering new insights to improve hoof health and animal welfare in modern dairy pastures.

Indexed as

Cattle DiseasesFoot DiseasesHoof and ClawQuercetinAnimal FeedAnimalsCattleDietDietary SupplementsFemaleLameness, AnimalNF-kappa BPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionNF-kappa BPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktQuercetinClaw lesionsDairy cowsInflammatory responseNF-κB signaling pathwayPI3K/Akt signaling pathwaysQuercetin

Identifiers

PMID41163003
PMCPMC12573863

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.