Evidence map›Paper›PMID 41166121›Full record

ArticleShock (Augusta, Ga.)2026

Chromatin Remodeling and Transcriptional Silencing Define the Dynamic Innate Immune Response of Tissue Resident Macrophages After Burn Injury.

Han G Kim, Marie-Pierre L Gauthier, Aidan Higgs, Denise A Hernandez, Mingqi Zhou, Jason O Brant, Rhonda L Bacher, Dijoia B Darden, Shannon M Wallet, Clayton E Mathews and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Shock (Augusta, Ga.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Han G KimDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Marie-Pierre L GauthierDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida.
Aidan HiggsNational Institute of Mental Health Intermural Research Program, National Institutes of Health, Bethesda, Maryland.
Denise A HernandezDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Mingqi ZhouDepartment of Biochemistry and Molecular Biology, College of Medicine, University of Florida, Gainesville, Florida.
Jason O BrantDepartment of Biostatistics, College of Public Health & Health Professions, University of Florida, Gainesville, Florida.
Rhonda L BacherDepartment of Biostatistics, College of Public Health & Health Professions, University of Florida, Gainesville, Florida.
Dijoia B DardenDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Shannon M WalletDepartment of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida.
Clayton E MathewsDepartment of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, Florida.
Lyle L MoldawerDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Philip A EfronDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Michael P KladdeDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.
Robert MaileDepartment of Surgery, Sepsis and Critical Illness Research Center, College of Medicine, University of Florida, Gainesville, Florida.

Funding

Renewal: Dysfunctional Myelopoiesis and Myeloid-Derived Suppressor Cells in Sepsis PathobiologyRM1GM139690 · NIGMS · UNIVERSITY OF FLORIDA · PI Philip A Efron, Michael Paul Kladde · 2021 to 2026
$10.3M
Resolving single-cell analysis challenges via data-driven decision frameworks and novel statistical methodsR35GM146895 · NIGMS · UNIVERSITY OF FLORIDA · PI Rhonda Bacher · 2022 to 2026
$1.9M
Multi-modal rescue of pulmonary NRF2-insufficiency after burn and burn + inhalation injury to regulate innate immune dysfunctionR01GM146134 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Robert Maile · 2022 to 2026
$1.6M
NIGMS NIH HHS R01 GM146134NIGMS NIH HHS R35 GM146895NIGMS NIH HHS RM1 GM139690
6 · The paper itself

Abstract

Severe burn injury induces prolonged immune dysfunction, but the underlying molecular mechanisms remain poorly defined. We hypothesized that burn injury causes epigenetic and transcriptional training of innate immune cells. Splenic F4/80⁺ macrophages were isolated from mice at 2, 9, and 14 days after 20% total body surface area contact burn. Targeted transcriptomics and chromatin profiling revealed a biphasic response: early transcriptional silencing of inflammatory genes (e.g., Stat3 , Traf6 , and Nfkb1 ), followed by increased accessibility and expression of anti-inflammatory loci ( Il-10 and Socs3 ). Metabolic genes showed persistent suppression of mitochondrial and oxidative phosphorylation programs. Canonical pathway analysis indicated early interleukin-10 signaling activation and long-term repression of classical macrophage activation. Chromatin remodeling included nucleosome repositioning events, supporting dynamic, and locus-specific regulation. These findings challenge the notion that burn-induced immune suppression is solely due to systemic inflammation and instead suggest durable, epigenetically programmed alterations in macrophage function.

Indexed as

BurnsChromatin Assembly and DisassemblyGene SilencingImmunity, InnateMacrophagesAnimalsInterleukin-10MaleMiceMice, Inbred C57BLSuppressor of Cytokine Signaling 3 ProteinInterleukin-10Suppressor of Cytokine Signaling 3 ProteinBurn Injuryepigeneticsimmune trainingmacrophages

Identifiers

PMID41166121
PMCPMC13245231

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.