Block sparse Bayes-based fuzzy system for RNA N6-methyladenosine sites prediction.

N6-methyladenosine (m6A) can significantly affect RNA expression, gene regulation, and determination of cell fate. As a common and abundant post-transcriptional modification (PTM) of RNA, m6A is also closely associated with the occurrence of numerous diseases. Thus, identifying the m6A modification site in the RNA sequence is a prerequisite for related research. High-throughput sequencing technology has high requirements and low cost performance. Computational methods have made encouraging progress in site prediction. However, most models only consider the effects of different species, ignoring the simultaneous exploration of RNA modifications in different tissues within the same species. We develop and validate a fuzzy system based on Block Sparse Bayesian Learning (BSBL), named BSBL-TSK-FS, which is a powerful sequence-level m6A prediction model. We introduce a Bayesian method that provides a posterior probability output to produce more sparse solutions so that the model has higher accuracy. The model classifies the m6A sites in several tissues of mouse, human, and rat. Under the five-fold cross-validation method (5-CV), the precision of the BSBL-TSK-FS model is 0.84∼0.95. The accuracy of our model improves by 9.4% over the existing SOTA predictors. BSBL-TSK-FS achieves superior performance over current SOTA methods. Finally, in order to verify the generalizability of the model, we carry out cross-species tests, and the results prove the robustness and adaptability of the model. An accurate and reliable sequence modification prediction model is developed to better understand the complex landscape of methylation modification.