Evidence map›Paper›PMID 41174698›Full record

ArticleStem cell research & therapy2025

Isolation and characterisation of AFP

Shuangliang Sun, Tegshjargal Badamjav, Namur Narid, Urjims Xu, Dunfu Suo, Batbold Batsaikhan, Bayar Hexig

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shuangliang Sun *State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Science, Inner Mongolia University, Hohhot, 010031, Inner Mongolia Autonomous Region, China.
Tegshjargal Badamjav *State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Science, Inner Mongolia University, Hohhot, 010031, Inner Mongolia Autonomous Region, China.
Namur NaridState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Science, Inner Mongolia University, Hohhot, 010031, Inner Mongolia Autonomous Region, China.
Urjims XuState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Science, Inner Mongolia University, Hohhot, 010031, Inner Mongolia Autonomous Region, China.
Dunfu SuoGraduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210, Mongolia.
Batbold BatsaikhanDepartment of Internal Medicine, Institute of Medical Sciences, Ulaanbaatar, 15160, Mongolia.
Bayar HexigState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Science, Inner Mongolia University, Hohhot, 010031, Inner Mongolia Autonomous Region, China. bhexig@imu.edu.cn.ORCID http://orcid.org/0000-0003-0280-1170

Funding

Major Projects of Science and Technology of Inner Mongolia Autonomous Region Grant No. zdzx2018044National Natural Science Foundation of China NSFC: Grand No. 31760267
6 · The paper itself

Abstract

backgroundThe liver is an organ with a strong regenerative capacity. Currently, partial hepatectomy (PHx) remains the most effective treatment option within various therapeutic strategies for liver diseases. When the liver undergoes acute injury, such as PHx, the remaining hepatocytes become hypertrophic, re-enter the cell cycle, initiate proliferation, and restore liver function. However, identifying the role of adult hepatic stem/progenitor cells (HSPCs) in this repair process remains challenging.

methodsIn this study, liver fibrosis was induced in mice by subcutaneous injection of carbon tetrachloride (CCl

resultsGene expression analysis revealed that the higher concentration of AFP⁺/DLK1⁺ double-positive cells was in the 50-70% Percoll layer, isolated via density gradient differential centrifugation. Pre-injection of CCl

conclusionsAFP and DLK1 serve as potential markers for selecting HSPC-like populations from the fibrotic PHx model. Our findings indicate that the fibrotic liver exhibits the most vigorous regenerative activity at 48 h after PHx, making it the optimal period for the isolation and extraction of HSPC-like cells which refers only to marker expression and proliferation, the relevance of the findings presented herein remains to be further verified.

Indexed as

alpha-FetoproteinsIntercellular Signaling Peptides and ProteinsLiver CirrhosisStem CellsAnimalsCalcium-Binding ProteinsCarbon TetrachlorideCell ProliferationDisease Models, AnimalHepatectomyHepatocytesLiverLiver RegenerationMaleMiceMice, Inbred C57BLalpha-FetoproteinsCalcium-Binding ProteinsCarbon TetrachlorideDlk1 protein, mouseIntercellular Signaling Peptides and ProteinsAlpha fetoproteinDelta-like noncanonical notch ligand 1 (DLK1)Hepatic stem/progenitor cells (HSPCs)Liver fibrosisPartial hepatectomy

Identifiers

PMID41174698
PMCPMC12577124

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.