Evidence map›Paper›PMID 41188804›Full record

ArticleBMC pulmonary medicine2025

Detection of viable Staphylococcus aureus/methicillin-resistant Staphylococcus aureus and antimicrobial resistance directly by PMA-TaqMan qPCR from bronchoalveolar lavage fluid.

Jun Luo, Min Liu, Wen Ai, Xiaoling Zheng, Lu Han, Kuo Huang, Changlin Zhang, Peng Wang, Jinhong Fan, Qianyuan Li and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pulmonary medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jun LuoThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China. lj1988cby@126.com.
Min LiuThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Wen AiThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Xiaoling ZhengThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Lu HanThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Kuo HuangThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Changlin ZhangThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Peng WangThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Jinhong FanThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Qianyuan LiThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Chunhua LuoThe First College of Clinical Medical Science, China Three Gorges University, Yichang, China. lchlgj2004@aliyun.com.

Funding

Hubei Provincial Natural Science Foundation of China 2023AFB652
6 · The paper itself

Abstract

backgroundTimely detection of viable Staphylococcus aureus (MSSA)/ methicillin-resistant Staphylococcus aureus (MRSA) and antimicrobial resistance are crucial for the treatment of patients with pulmonary infection.

methodsIn the current study, we have developed and evaluated the potential clinical applicability of the PMA TaqMan-based real-time PCR (PMA-TaqMan qPCR) assay for detection of viable MSSA/MRSA and antimicrobial susceptibility directly from bronchoalveolar lavage fluid.

resultsThe PMA-TaqMan qPCR assay demonstrated high sensitivity with a detection limit of 800 CFU/mL within 115 min. Furthermore, the assay required only 45 min of antibiotic exposure and completed antimicrobial susceptibility testing within 160 min. Upon detection of 10 spiked bronchoalveolar lavage fluid samples treated by clindamycin after 24 h, the PMA-TaqMan qPCR assay showed better agreement with culture-based methods in detecting viable bacteria. Subsequent antimicrobial susceptibility results of bronchoalveolar lavage fluid samples spiked by 16 MRSA isolates and 11 MSSA isolates based on the current assay, compared with the standard broth microdilution method, showed an overall agreement of 100% (95% confidence intervals [CI]: 80.64 to 100) for MRSA and 90.91% (95% CI: 62.27 to 98.38) for MSSA.

conclusionsOur findings demonstrate that the PMA-TaqMan qPCR assay is a highly sensitive and specific method for the direct detection of viable MSSA/MRSA and antimicrobial susceptibility testing from bronchoalveolar lavage fluid.

Indexed as

Bronchoalveolar Lavage FluidMethicillin-Resistant Staphylococcus aureusReal-Time Polymerase Chain ReactionStaphylococcus aureusAnti-Bacterial AgentsClindamycinHumansMicrobial Sensitivity TestsSensitivity and SpecificityAnti-Bacterial AgentsClindamycinBroad-spectrum antimicrobial susceptibilityMSSA/MRSA identificationPMA-TaqMan qPCR assayPulmonary infectionViable bacteria detection

Identifiers

PMID41188804
PMCPMC12584419

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.