Evidence map›Paper›PMID 41194921›Full record

ArticleFrontiers in immunology2025

Phospholipase D1 is a critical mediator of neutrophil extracellular trap formation and venous thrombosis.

Ryosuke Aihara, Masatomo Takahashi, Kenji Morino, Keisuke Matsubara, Kazufumi Kunimura, Akihiko Nishikimi, Yoshihiro Izumi, Takeshi Bamba, Yoshinori Fukui, Takehito Uruno

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ryosuke AiharaDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Masatomo TakahashiDivision of Metabolomics, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Kenji MorinoDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Keisuke MatsubaraDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Kazufumi KunimuraDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Akihiko NishikimiBiosafety Administration Division, Research Institute, National Center for Geriatrics and Gerontology, Aichi, Japan.
Yoshihiro IzumiDivision of Metabolomics, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Takeshi BambaDivision of Metabolomics, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Yoshinori FukuiDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
Takehito UrunoDivision of Immunogenetics, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neutrophil extracellular traps (NETs) are a host defense mechanism whereby activated neutrophils release decondensed chromatin and antimicrobial proteins into the extracellular space to trap and kill invading pathogens. While effective in clearing pathogens, NETs also pose pathological risks by exposing self-DNA, histones, granular enzymes, and reactive oxygen species (ROS), contributing to pathologies such as autoimmune diseases, inflammatory disorders, and thrombosis. Here, we identify phospholipase D1 (PLD1), a lipid-signaling enzyme that generates phosphatidic acid (PA), as a critical regulator of ROS generation and NET formation in murine neutrophils. Using both PLD1-deficient neutrophils and a selective inhibitor, we demonstrate that PLD1 is essential for NET release. Notably, exogenous PA alone is sufficient to trigger robust ROS production and NET formation.

Indexed as

Extracellular TrapsNeutrophilsPhospholipase DVenous ThrombosisAnimalsDisease Models, AnimalHumansMiceMice, Inbred C57BLMice, KnockoutPhosphatidic AcidsReactive Oxygen SpeciesPhosphatidic AcidsPhospholipase Dphospholipase D1Reactive Oxygen Speciesdeep vein thrombosisneutrophil extracellular trapphosphatidic acidphospholipase D1reactive oxygen species

Identifiers

PMID41194921
PMCPMC12583085

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.