Evidence mapPaperPMID 41196279Full record

ArticleImmunology2026

Tui Na Acupressure Modulates Treg Immunosuppression via FoxP3/mTORC1 Signalling in ALS Mice.

Jie Han, Xi Xu, Yan Zhao, Yao Xiao, Fei Huang, Jing Zhou, Hai Huang, Guiqian Wang

Abstract read
In one paragraph

Article in Immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jie HanRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Xi XuAffiliated Hospital of Hubei University of Chinese Medicine, Wuhan, China.ORCID 0000-0002-0691-7077
Yan ZhaoRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Yao XiaoRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Fei HuangRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Jing ZhouRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Hai HuangRehabilitation Medicine Center/Tuina Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Guiqian WangAffiliated Hospital of Hubei University of Chinese Medicine, Wuhan, China.

Funding

Development Joint Fund Project 2023AFD128Development Joint Fund Project 2023AFD170Development Joint Fund Project 2023AFD171Development Joint Fund Project 2025AFD535Hubei Provincial Natural Science Foundation's InnovationScience and Technology Special Project of the State Administration of Traditional Chinese Medicine GZY-KJS-2025-008
6 · The paper itself

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease driven by neuroinflammation, where regulatory T cell (Treg) dysfunction exacerbates immune imbalance. This study explores whether Tui Na acupressure, a traditional Chinese therapy, can restore Treg immunosuppressive function through the FoxP3/mTORC1 signalling pathway to mitigate ALS pathology. In SOD1G93A ALS mice, Tui Na was applied at the Shenshu acupoint, with motor and cognitive functions assessed via rotarod, tail suspension, novel object recognition, and Y-maze tests. Multi-omics (transcriptomics, proteomics), flow cytometry, ELISA, and Western blot analysed Treg proportions, cytokine profiles, and pathway activation. In vitro assays evaluated Treg proliferation and immunosuppression. Tui Na significantly enhanced motor and cognitive performance, increased Treg proportions in spleen, lymph nodes, and blood, and elevated anti-inflammatory cytokines (IL-10, TGF-β) while reducing pro-inflammatory markers (IL-6, TNF-α). Transcriptomic and proteomic analyses revealed upregulated FoxP3, Mtor, and Raptor, with enhanced Treg proliferation and immunosuppression confirmed in vitro. Pathway inhibitors (GSK126, rapamycin) reversed these effects, confirming FoxP3/mTORC1 dependency. Tui Na also reduced apoptosis and oxidative stress, supporting immune regulation. These findings highlight Tui Na's potential to restore Treg-mediated immune balance in ALS, offering a non-pharmacological therapeutic strategy. This study provides novel immunological insights into Tui Na's mechanisms, advocating its clinical evaluation for ALS and related immune-driven disorders.

Indexed as

AcupressureAmyotrophic Lateral SclerosisForkhead Transcription FactorsMechanistic Target of Rapamycin Complex 1T-Lymphocytes, RegulatoryAnimalsCell ProliferationCytokinesDisease Models, AnimalHumansImmune ToleranceMaleMiceMice, TransgenicSignal TransductionCytokinesForkhead Transcription FactorsFoxp3 protein, mouseMechanistic Target of Rapamycin Complex 1amyotrophic lateral sclerosisFoxP3/mTORC1 pathwayimmunosuppressionneuroinflammationregulatory T cellsTui Na acupressure

Identifiers

PMID41196279
PMCPMC12867591

What Socratic holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.