ArticleArchives of virology2025
Virucidal activity of Cannabis sativa L. (hemp) root and stem extracts against Japanese encephalitis virus: role of stigmasterol.
Article in Archives of virology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Magnolol Modulates the RIG-I/NF-κB Signaling Pathway to Alleviate JEV Infection-Induced ST Cell Damage.Animals : an open access journal from MDPI · 2026Article
- A plant gallotannin pentagalloyl d-glucose elicits antiviral activity against influenza A and B viruses through multi-targeting mechanisms.Archives of virology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Japanese encephalitis virus (JEV) poses a significant public health risk due to the lack of effective antiviral therapies. To identify novel antiviral agents, we evaluated the antiviral activity of ethanol extracts and organic solvent fractions derived from the roots and stems of hemp (Cannabis sativa L.). Noncytotoxic concentrations of the extracts and fractions were determined using in vitro cytotoxicity assays. At these concentrations, several fractions demonstrated potent virucidal activity, with the hexane and chloroform fractions showing the strongest effects. Post-treatment of virus-infected cells with these fractions significantly suppressed viral replication, as evidenced by reduced JEV mRNA and E protein expression. In contrast, pre-treatment or co-treatment did not yield notable antiviral effects. GC-MS analysis revealed the presence of multiple known hemp-derived compounds in the active fractions. Among them, stigmasterol exhibited strong virucidal and antiviral activity. It inhibited viral entry and growth when applied during or after infection and significantly decreased viral mRNA and E protein levels in infected cells. These findings suggest that stigmasterol contributes to the antiviral effects of hemp extracts and may be one of the active compounds responsible for inhibiting JEV replication. This study highlights the potential of hemp-derived natural products, particularly stigmasterol, as promising candidates for the development of antiviral agents against JEV infection.
Indexed as
Identifiers
41196377What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.