Evidence map›Paper›PMID 41196420›Full record

ArticleMolecular biology reports2025

Imperatorin ameliorates renal damage and modulates MAPK, Nrf2, and NF-kB pathways in mercury-chloride-exposed rats: A toxicological study relevant to skin lightening practices.

Reshma Murali, Abilash Valsala Gopalakrishnan

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Reshma MuraliDepartment of Biomedical Sciences, School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India.
Abilash Valsala GopalakrishnanDepartment of Biomedical Sciences, School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632014, Tamil Nadu, India. abilash.vg@vit.ac.in.ORCID http://orcid.org/0000-0003-0780-0492

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA major global public health concern is the widespread and illegal use of mercury compounds in skin-lightening cosmetics, mainly because of their extreme systemic toxicity, especially to the kidneys. Imperatorin is a promising natural furanocoumarin compound with high antioxidant properties. This study investigates the mechanism of mercury-induced nephrotoxicity and the curative properties of imperatorin, focusing on the NF-κB, MAPKs, and Nrf2 signalling pathways.

methodsRats were divided into control, mercury-chloride (HgCl2)-treated (2.5 mg/kg), imperatorin-treated (15 mg/kg), and two co-treated groups receiving HgCl2 (2.5 mg/kg) with either 10 mg/kg or 15 mg/kg of imperatorin. Our study examined tissue histopathology, renal function markers, oxidative stress parameters, inflammatory cytokines, as well as the expression of nephrin, podocin, Kidney Injury Marker-1 (KIM-1), Nrf2, NF-kB, p38, ERK, and JNK mRNA levels. Further, the activated protein levels of p38, ERK and JNK were confirmed using a western blot.

resultsImperatorin decreased the levels of creatinine, urea, and uric acid; increased protein and albumin levels and restored pathological changes in a dose-dependent manner. The antioxidant levels of superoxide dismutase, reduced glutathione, glutathione peroxidase and catalase were also restored by imperatorin treatment while modulating the levels of ROS, MDA, TNF-α, IL-6 and IL-1β. Gene expression analysis showed a significant increase in KIM-1 and a decrease in nephrin and podocin levels in the HgCl2 group, suggesting kidney injury. Imperatorin modulated the gene expression levels of MAPKs (p38, Erk, and Jnk), Nrf2, and NF-κB in the HgCl2 groups. Protein expression levels of MAPKs confirmed that MAPK signal activation in the HgCl2 group decreased by imperatorin treatment.

conclusionThese results show that in an in vivo model, imperatorin provides significant protection against HgCl2-induced nephrotoxicity.

Indexed as

FurocoumarinsKidney DiseasesMercuric ChlorideNF-E2-Related Factor 2AnimalsAntioxidantsKidneyMaleMAP Kinase Signaling SystemMitogen-Activated Protein KinasesNF-kappa BOxidative StressRatsSignal TransductionAntioxidantsFurocoumarinsimperatorinMercuric ChlorideMitogen-Activated Protein KinasesNfe2l2 protein, ratNF-E2-Related Factor 2NF-kappa BImperatorinMAPKMercuryNephrotoxicitySkin lightening products

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