Evidence map›Paper›PMID 41199599›Full record

ArticleRenal failure2025

The synergistic renoprotective effects of valproate sodium and metformin in a diabetic nephropathy mouse model: attenuation of pro-inflammatory markers and enhancement of Sirt1 and Bcl-2 expression.

Parisa Saberi-Hasanabadi, Hossein Ghalhenoi, Roghayeh Jahani, Sepideh Saberi, Seyed Mohammad Reza Sayedi Moqadam, Fereshteh Talebpour Amiri, Ramin Ataee

Abstract read
In one paragraph

Article in Renal failure, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Frontiers in pharmacology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Parisa Saberi-HasanabadiDepartment of Toxicology and Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran.
Hossein GhalhenoiDepartment of Medical Biotechnology, Mazandaran University of Medical Sciences, Sari, Iran.
Roghayeh JahaniDepartment of Toxicology and Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran.
Sepideh SaberiStudent Research Committee, Islamic Azad University, Tonekaban, Iran.
Seyed Mohammad Reza Sayedi MoqadamDepartment of Toxicology and Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran.
Fereshteh Talebpour AmiriMolecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Ramin AtaeeMedicinal Plants Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diabetic nephropathy is a serious and common complication among patients with both type 1 and type 2 diabetes, and it significantly reduces the patient's quality of life. This study aimed to assess the reno preventive effects of valproate sodium (VPS) and metformin (MET) on alloxan-induced diabetic nephropathy and to elucidate their mechanisms of action, 'type 1 diabetic mice' (25-30 g) were established using a single dose of alloxan ('120 mgkg-1'). and the diabetic mice were treated with three doses of VPS (10, 20, and 40 mg/kg) and MET (200 mg/kg) for a period of 28 days. Specific tests were performed to evaluate inflammatory gene expression (TNF-α, IL-6, and NF-κB) and histopathological changes and apoptotic factors (Bax/Bcl2, Caspase3). Our results have shown, VPS and MET led to significant decreases in blood glucose levels, thereby reflecting the improvement of impaired kidney function and decreasing elevated renal mRNA levels of inflammatory genes (TNF-α, IL-6, and NF-κB) in diabetic mice. A significant increase in the expression of be 'Sirt1 and Bcl-2' and decrease in (TNF-α, IL-6, and NF-κB) was observed in the kidneys of diabetic mice receiving MET/VPS Moreover, MET/VPS successfully prevented diabetes induced 'histopathological deleterious changes' in the kidneys of mice so it can concluded that MET and VPS alone or in combination can prevent alloxan-induced diabetic nephropathy through attenuating inflammatory markers and probably with suppression of apoptosis.

Indexed as

Diabetes Mellitus, ExperimentalDiabetic NephropathiesHypoglycemic AgentsMetforminValproic AcidAnimalsApoptosisBiomarkersBlood GlucoseDisease Models, AnimalDrug SynergismDrug Therapy, CombinationInterleukin-6KidneyMaleMiceBcl2 protein, mouseBiomarkersBlood GlucoseHypoglycemic AgentsInterleukin-6MetforminNF-kappa BProto-Oncogene Proteins c-bcl-2Sirt1 protein, mouseSirtuin 1Tumor Necrosis Factor-alphaValproic Acidalloxananti-inflammatoryDiabetesdiabetic nephropathymetforminvalproate sodium

Identifiers

PMID41199599
PMCPMC12599348

What Socratic holds

Texttitle and abstract
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.