Evidence map›Paper›PMID 41200307›Full record

ArticleOpen veterinary journal2025

Increasing the fertility of Sapudi sheep semen at 5°C storage temperature with the addition of oxytocin in a diluent of skim milk, egg yolk, and citrate.

Sri Mulyati, Imam Mustofa, Suherni Susilowati, Aswin Rafif Khairullah, Riza Zainuddin Ahmad, Adeyinka Oye Akintunde, Bima Putra Pratama, Latifah Latifah, Ulvi Fitri Handayani, Lili Anggraini

Abstract read
In one paragraph

Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sri MulyatiDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Imam MustofaDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Suherni SusilowatiDivision of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Aswin Rafif KhairullahResearch Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Riza Zainuddin AhmadResearch Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Adeyinka Oye AkintundeDepartment of Agriculture and Industrial Technology, Babcock University, Ilishan Remo, Nigeria.
Bima Putra PratamaResearch Center for Agroindustry, National Research and Innovation Agency (BRIN), South Tangerang, Indonesia.
Latifah LatifahResearch Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Ulvi Fitri HandayaniResearch Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia.
Lili AnggrainiResearch Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Bogor, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Artificial insemination in sheep is one of the important reproductive technologies that accelerate the increase in the population of Sapudi sheep. The success of fertilization is higher with the addition of oxytocin in semen than with exogenous oxytocin administration. The semen diluents used were milk and egg yolk citrate. Aim: This study aimed to determine the effect of oxytocin inclusion in extender on viability, motility, plasma membrane integrity, malondialdehyde (MDA) levels, and catalase levels in Sapudi sheep semen stored at 5°C. Methods: Semen was collected with an artificial vagina and examined macroscopically and microscopically. Each ejaculate was divided into four parts according to the treatment group. The control group (T0) consisted of fresh semen diluted in a skim milk-egg yolk-citrate extender without oxytocin addition. The treatment groups received the same extender supplemented with oxytocin at different concentrations: T1 with 5 IU/100 ml, T2 with 10 IU/100 ml, and T3 with 15 IU/100 ml of extender. All treatments were stored at 5°C and evaluated daily for 5 days. Results: Group T2 had the highest viability, motility, intact plasma membrane, and catalase enzyme levels ( Conclusion: This study indicated that the inclusion of 10 IU/100 ml of oxytocin in skim milk-egg yolk-citrate extender can maintain the quality of Sapudi ram semen with a motility of more than 40% after 5 days of refrigerated storage.

Indexed as

OxytocinSemenSemen PreservationAnimalsCitric AcidEgg YolkFertilityMaleMilkSheepSheep, DomesticSperm MotilityCitric AcidOxytocinCatalaseExtendersMotilityUndernutritionViability

Identifiers

PMID41200307
PMCPMC12587903

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.