RM2Target v2.0: an updated database for the target genes of writers, erasers, and readers of RNA modifications.

Defining regulatory associations between RNA modification proteins [writers, erasers, and readers (WERs)] and their target genes is crucial for elucidating the molecular mechanisms underlying RNA modifications. We previously developed RM2Target, a comprehensive database of WER-target associations across nine RNA modification types in humans and mice, which has become a widely utilized resource in the field. However, the growing body of research on novel RNA modifications and non-model species has created an urgent need for a significant expansion of the database. Here, we introduce RM2Target v2.0 (http://rm2target.canceromics.org/), an updated version featuring substantially expanded data coverage and enhanced curation. The new release includes 4 400 616 WER-target associations, encompassing 273 WER proteins from 18 RNA modification types and spanning 973 cell lines or tissues across 20 species. Additionally, RM2Target v2.0 provides extensive annotations for target genes, including basic gene information, associated RNA modifications, RNA-RNA and RNA-protein interactions, and disease linkages. We expect that RM2Target v2.0 will serve as a foundational resource for exploring RNA epitranscriptomic regulation, enabling investigations into cross-talk among modifications, underlying molecular mechanisms, and disease connections, thereby facilitating both basic research and translational applications in RNA epigenetics.