Evidence mapPaperPMID 41212388Full record

ArticleJournal of assisted reproduction and genetics2026

Downregulated miR-363-5p causes recurrent spontaneous abortion (RSA) by regulating the S100A1 expression.

Guimei Zhang, Yingxuan Huang, Lin Zheng, Shihui Hao, Jingyun Cao, Liqin Zhang

Abstract read
In one paragraph

Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Guimei Zhang *Department of Gynecology and Obstetrics, Anze Medical Group Traditional Chinese Medicine Hospital, Linfen, 042500, China.
Yingxuan Huang *Key Laboratory of Research and Development On Clinical Molecular Diagnosis for High-Incidence Diseases of Baise, Baise, Guangxi, 533000, China.
Lin ZhengGynaecology, Tonglu County Traditional Chinese Medicine Hospital, Hangzhou, 311500, China.
Shihui HaoDepartment of Obstetrics and Gynecology, Liwan Central Hospital of Guangzhou, Guangzhou, 510180, China.
Jingyun CaoDepartment of Fertility Technology, Changzhou Maternal and Child Health Care Hospital, Changzhou Medical Center, Nanjing Medical University, Changzhou, 213000, China.
Liqin ZhangDepartment of Gynecology and Obstetrics, Gusu School, Nanjing Medical University, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, 26 Daoqian Street, Gusu District, Suzhou, 215002, China. Zhangliqin8711@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeRecurrent spontaneous abortion (RSA) involves complex pathophysiology, making it difficult to develop effective treatments and causing significant health and economic burdens. This study evaluated miR-363-5p as a potential biomarker for RSA and explored its role in disease progression to provide insights for clinical management.

methodsA total of 68 serum samples from RSA patients and 97 from healthy pregnant controls were analyzed to determine miR-363-5p expression and its clinical significance. The EVT cell line HTR-8/SVneo was used for in vitro experiments. Cell proliferation was assessed using CCK-8 assays, while Transwell assays evaluated migration and invasion. qRT-PCR detected miR-363-5p and S100A1 expression levels. Dual-luciferase reporter assays confirmed the interaction between miR-363-5p and S100A1.

resultsmiR-363-5p expression was significantly lower in RSA patients than in healthy pregnant controls. ROC analysis indicated its high diagnostic potential for RSA. In vitro experiments showed that miR-363-5p promoted HTR-8/SVneo cell proliferation, migration, and invasion while inhibiting apoptosis. S100A1 was identified as a direct target of miR-363-5p in RSA. miR-363-5p regulates EVT cell functions by suppressing S100A1 expression.

conclusionsSerum miR-363-5p downregulation may serve as a diagnostic biomarker for RSA. miR-363-5p likely affects pregnancy outcomes in RSA by targeting S100A1 to regulate EVT cell functions. These findings suggest that miR-363-5p has potential for both diagnosing and treating RSA.

Indexed as

Abortion, HabitualMicroRNAsS100 ProteinsAdultApoptosisBiomarkersCell LineCell MovementCell ProliferationDown-RegulationFemaleGene Expression RegulationHumansPregnancyTrophoblastsBiomarkersMicroRNAsMIRN363 microRNA, humanS100 ProteinsCell behaviorDiagnostic markerMiR-363-5pRecurrent spontaneous abortionS100A1

Identifiers

PMID41212388
PMCPMC12901758

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.