Evidence map›Paper›PMID 41214365›Full record

ArticleEMBO reports2025

Functional profiling and visualization of the sphingolipid metabolic network in vivo.

Fei-Yang Tzou, Cheng-Li Hong, Kai-Hung Chen, John P Vaughen, Wan-Syuan Lin, Chia-Heng Hsu, Irma Magaly Rivas-Serna, Kai-Yi Hsu, Shuk-Man Ho, Michael Raphael Panganiban and 13 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in EMBO reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors.

Fei-Yang TzouGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.ORCID http://orcid.org/0000-0003-3463-1436
Cheng-Li HongGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.ORCID http://orcid.org/0009-0008-5679-5013
Kai-Hung ChenGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
John P VaughenDepartment of Anatomy, University of California San Francisco, CA, 94114, USA.
Wan-Syuan LinGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Chia-Heng HsuGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Irma Magaly Rivas-SernaDepartment of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2R3, Canada.
Kai-Yi HsuInstitute of Systems Neuroscience, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Shuk-Man HoDepartment of Biochemistry and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.
Michael Raphael PanganibanInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei, 11529, Taiwan.ORCID http://orcid.org/0000-0001-8012-1061
Hsin-Ti HsiehInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei, 11529, Taiwan.
Yi-Jhan LiGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.ORCID http://orcid.org/0009-0004-6321-6244
Yi HsiaoGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.ORCID http://orcid.org/0009-0000-2528-0844
Hsin-Chun YehGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
Cheng-Yu YuInterdisciplinary Program of Life Sciences and Medicine, National Tsing-Hua University, Hsinchu, 300044, Taiwan.
Hong-Wen TangProgram in Cancer and Stem Cell Biology, Duke-NUS Medical School, 8 College Road, Singapore, 169857, Singapore.ORCID http://orcid.org/0000-0002-8347-9891
Ya-Hui ChouInstitute of Cellular and Organismic Biology, Academia Sinica, Taipei, 11529, Taiwan.ORCID http://orcid.org/0000-0001-6552-8728
Chia-Lin WuDepartment of Biochemistry and Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan.ORCID http://orcid.org/0000-0002-7906-2364
Chung-Chuan LoInstitute of Systems Neuroscience, National Tsing Hua University, Hsinchu, 300044, Taiwan.
Vera C MazurakDepartment of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2R3, Canada.
M Thomas ClandininDepartment of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2R3, Canada.
Shu-Yi HuangDepartment of Medical Research, National Taiwan University Hospital, Taipei, 100, Taiwan.ORCID http://orcid.org/0000-0002-3522-0500
Chih-Chiang ChanGraduate Institute of Physiology, College of Medicine, National Taiwan University, Taipei, 100, Taiwan. chancc1@ntu.edu.tw.ORCID http://orcid.org/0000-0003-2626-3805

Funding

National Health Research Institutes (NHRI) EX114-11228NINational Health Research Institutes (NHRI) NHRI-13A1-CG-CO-08-2325-2National Science and Technology Council (NSTC) 112-2311-B-001-036-MY3National Science and Technology Council (NSTC) 113-2320-B-002-022-MY3National Science and Technology Council (NSTC) 114-2311-B-002-006-National Taiwan University () 114L8522National Taiwan University () 114L891303National Taiwan University () 114L910203National Taiwan University Hospital (NTUH) 113-L3003
6 · The paper itself

Abstract

Sphingolipids govern diverse cellular processes; their dysregulation underlies numerous diseases. Despite extensive characterizations, understanding the orchestration of the sphingolipid network within living organisms remains challenging. We established a versatile genetic platform of CRISPR-engineered reporters of 52 sphingolipid regulators, recapitulating endogenous gene activity and protein distribution. This platform further allows conditional protein degradation for functional characterization. In addition, we developed the biosensor OlyA

Indexed as

Metabolic Networks and PathwaysSphingolipidsAnimalsBrainCRISPR-Cas SystemsEthanolaminesHumansMembrane MicrodomainsMiceSphingomyelin PhosphodiesteraseSphingomyelinsceramide phosphoethanolamineEthanolaminesSphingolipidsSphingomyelin PhosphodiesteraseSphingomyelinsBrainCell-type SpecificitySpatial HeterogeneitySphingolipidsSystemic Profiling

Identifiers

PMID41214365
PMCPMC12714868

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.