Evidence map›Paper›PMID 41214720›Full record

ArticleJournal of orthopaedic surgery and research2025

RIPK3 is spatially associated with cartilage degeneration in osteoarthritis: integrative transcriptomic and histological analysis.

Lei Wang, Jing Tang, Lei Niu, Zihua Li, Lang Wu, Wei Zhao, Guanghui Wang

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Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Lei Wang *Department of Trauma and Joint Surgery, The Fifth People's Hospital of Ningxia Hui Autonomous Region, Shizuishan, 753000, Ningxia, China.
Jing Tang *School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China.
Lei NiuSchool of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China.
Zihua LiSchool of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China.
Lang WuDepartment of Hepatobiliary Surgery, The People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, 750000, China.
Wei ZhaoSchool of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China. zw-6915@163.com.
Guanghui WangDepartment of Trauma and Joint Surgery, The Fifth People's Hospital of Ningxia Hui Autonomous Region, Shizuishan, 753000, Ningxia, China. guanghui03160@163.com.

Funding

Ningxia Hui Autonomous Region Key Research Program 2022BEG03156
6 · The paper itself

Abstract

backgroundOsteoarthritis (OA) is a degenerative joint disease characterized by progressive cartilage loss, yet its molecular underpinnings remain incompletely defined. This study aimed to investigate the regional expression pattern of receptor-interacting protein kinase 3 (RIPK3) in OA cartilage and its association with histopathological severity.

methodsCartilage samples were collected from OA patients and categorized into normal tissue (NT), junction tissue (JT), and lesioned tissue (LT). RNA sequencing was performed to identify differentially expressed genes (DEGs) between NT and JT. Gene enrichment analysis was conducted to explore functional pathways. Immunofluorescence staining was used to validate RIPK3 protein expression and localization.

resultsTranscriptomic analysis identified 140 differentially expressed genes (DEGs) between NT and JT, with several genes, including RIPK3, CCL19, and ITLN1, significantly up-regulated in JT. RIPK3 expression showed a log

conclusionsRIPK3 is spatially enriched in degenerative regions of OA cartilage and associates with histopathological damage, suggesting its potential involvement in disease progression. These findings provide new insight into the molecular landscape of OA and support RIPK3 for further evaluation as a biomarker and potential therapeutic target.

Indexed as

Cartilage, ArticularOsteoarthritisReceptor-Interacting Protein Serine-Threonine KinasesTranscriptomeAgedFemaleGene Expression ProfilingHumansMaleMiddle AgedReceptor-Interacting Protein Serine-Threonine KinasesRIPK3 protein, humanCartilage degenerationMulti-regional transcriptomicsOsteoarthritisRIPK3

Identifiers

PMID41214720
PMCPMC12604343

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.