Evidence mapPaperPMID 41214723Full record

ArticleVeterinary research2025

Transcriptomic landscape of pseudorabies virus-induced encephalitis reveals key lncRNAs involved in host-neurotropic virus interactions.

Thach Phan Van, Tien Huyen Ton Nu Bao, Byungkwan Oh, Ngan Tran Thi Kim, Sang-Ik Oh, Bumseok Kim

Abstract read
In one paragraph

Article in Veterinary research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Animal virus-host interactions mediated by non-coding RNAs.Frontiers in cellular and infection microbiology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Thach Phan VanLaboratory of Veterinary Pathology and Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Tien Huyen Ton Nu BaoLaboratory of Veterinary Pathology and Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Byungkwan OhLaboratory of Veterinary Pathology and Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Ngan Tran Thi KimDepartment of Husbandry and Veterinary Medicine, School of Agriculture and Natural Resources, Vinh University, 182 Le Duan, Vinh, Nghe An, 460000, Vietnam.
Sang-Ik OhLaboratory of Veterinary Pathology and Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, Republic of Korea.
Bumseok KimLaboratory of Veterinary Pathology and Biosafety Research Institute, College of Veterinary Medicine, Jeonbuk National University, Iksan, 54596, Republic of Korea. bskims@jbnu.ac.kr.

Funding

This work was supported by the Basic Science Research Program and Leading University Project for International Cooperation through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (RS-2019-NR040075, NRF-2023H1A7A2A02000078)
6 · The paper itself

Abstract

Pseudorabies virus (PRV) infection causes fatal encephalitis across various species, a condition known as pseudorabies encephalitis (PRE). However, the molecular mechanisms underlying PRE remain poorly understood. Long noncoding RNAs (lncRNAs) have emerged as important regulators of gene expression in neurological diseases and viral infections. This study explores genome-wide transcriptional alterations in a mouse model of PRV-induced encephalitis. The intranasal inoculation of mice with PRV induced severe encephalitis, characterized by high viral loads, significant inflammatory responses, and the onset of neurological symptoms. RNA-seq analysis revealed 683 differentially expressed (DE) mRNAs and 179 DElncRNAs in PRV-infected brains compared with controls. Functional and pathway analyses revealed that PRE involves neurodegeneration and diverse immune responses, reflecting the complex interplay between viral evasion strategies and host defenses. Co-expression network analysis, supported by experimental validation, identified several lncRNAs as central hubs interacting with multiple immune-related genes and exhibiting cell type-specific expression pattern. Notably, ZFAS1 was prominently dysregulated in PRV-infected microglia and showed extensive co-expression connectivity. Knockdown of ZFAS1 modulated microglia-driven inflammation without altering viral replication, underscoring its potential as a therapeutic target for mitigating neuroinflammation in virus-associated neurological diseases. The identification of key lncRNAs and their potential regulatory roles deepens our understanding of disease mechanisms, while offering new avenues for therapeutic intervention in neurotropic viral infections.

Indexed as

Encephalitis, ViralHerpesvirus 1, SuidPseudorabiesRNA, Long NoncodingTranscriptomeAnimalsFemaleMiceMice, Inbred C57BLRNA, Long Noncodingencephalitisimmune responselong noncoding RNAPseudorabies virusRNA sequencing

Identifiers

PMID41214723
PMCPMC12604289

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.