Evidence map›Paper›PMID 41219199›Full record

ArticleNature communications2025

CryoEM and computational modeling structural insights into the pH regulator NBCn1.

Weiguang Wang, Hristina R Zhekova, Kirill Tsirulnikov, B Sridhar Dwadasi, Ehecatl Guzman Aparicio, Rustam Azimov, Natalia Abuladze, Liyo Kao, Dora Acuna, D Peter Tieleman and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Levetiracetam inhibits the NaActa physiologica (Oxford, England) · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Weiguang Wang *Department of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0003-4398-4444
Hristina R Zhekova *Centre for Molecular Simulation, Department of Biological Sciences, University of Calgary, Calgary, Canada.ORCID http://orcid.org/0000-0002-7022-2034
Kirill TsirulnikovDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
B Sridhar DwadasiCentre for Molecular Simulation, Department of Biological Sciences, University of Calgary, Calgary, Canada.ORCID http://orcid.org/0000-0001-8552-9285
Ehecatl Guzman AparicioCentre for Molecular Simulation, Department of Biological Sciences, University of Calgary, Calgary, Canada.ORCID http://orcid.org/0009-0008-8124-0394
Rustam AzimovDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Natalia AbuladzeDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Liyo KaoDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Dora AcunaDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.ORCID http://orcid.org/0009-0006-0148-8804
D Peter TielemanCentre for Molecular Simulation, Department of Biological Sciences, University of Calgary, Calgary, Canada.ORCID http://orcid.org/0000-0001-5507-0688
Z Hong ZhouElectron Imaging Center for Nanomachines, California NanoSystems Institute, University of California, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0002-8373-4717
Alexander PushkinDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Ira KurtzDepartment of Medicine, Division of Nephrology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA. ikurtz@mednet.ucla.edu.ORCID http://orcid.org/0000-0003-2834-510X

Funding

The Biology of NBCe1 in Health and DiseaseR01DK077162 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI IRA KURTZ · 2007 to 2026
$6.7M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
Breakthrough Molecular Dynamics Research via an Anton2 SupercomputerR01GM116961 · NIGMS · CARNEGIE-MELLON UNIVERSITY · PI BLOOD, PHILIP D. · 2016 to 2023
$3.0M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
NCRR NIH HHS S10 RR023057NIDDK NIH HHS R01 DK077162NIGMS NIH HHS R01 GM071940NIGMS NIH HHS R01 GM116961U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) DK077162
6 · The paper itself

Abstract

Breast cancer cells survive despite being exposed to a toxic acidic extracellular environment, by utilizing the NBCn1 transporter. The molecular basis for this phenomenon is unknown, given the lack of an NBCn1 atomic structural model. We therefore determined the 3.3 Å cryoEM structure of the human NBCn1 outward facing (OF) conformational state with densities corresponding to the transported ions in the ion coordination site. We further generated NBCn1 inward facing (IF) and intermediate (occluded) structures and characterized the transport cycle and the ion dynamics in the IF and OF states. The results showed that NBCn1 utilizes an elevator-type transport mechanism with a small vertical shift of the ion coordination site between OF and IF conformational states and that the transported ions permeate without significant energy barriers. Functional experiments showed that NBCn1 has an extremely high ion turnover rate (TOR) of ~15,000 s

Indexed as

Sodium-Bicarbonate SymportersBreast NeoplasmsCryoelectron MicroscopyHumansHydrogen-Ion ConcentrationIon TransportModels, MolecularProtein ConformationSLC4A7 protein, humanSodium-Bicarbonate Symporters

Identifiers

PMID41219199
PMCPMC12606367

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.