Evidence mapPaperPMID 41220186Full record

ArticleJournal of extracellular vesicles2025

Recommendations for Studying In Situ Extracellular Vesicles From Solid Tissue.

Rossella Crescitelli, Yiyao Huang, An Hendrix, Andrew F Hill, Stephanie N Hurwitz, Tsuneya Ikezu, Efrat Levy, Berta Puig, Lucia Paolini, Laura J Vella

Abstract read
In one paragraph

Article in Journal of extracellular vesicles, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rossella CrescitelliSahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID https://orcid.org/0000-0002-1714-3169
Yiyao HuangDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
An HendrixLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.
Andrew F HillInstitute for Health and Sport, Victoria University, Melbourne, Victoria, Australia.ORCID https://orcid.org/0000-0001-5581-2354
Stephanie N HurwitzDepartment of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Tsuneya IkezuDepartment of Neuroscience, Mayo Clinic Florida, Jacksonville, Florida, USA.ORCID https://orcid.org/0000-0002-3979-8596
Efrat LevyCenter for Dementia Research, Nathan S. Kline Institute for Psychiatric Research, Orangeburg, New York, USA.
Berta PuigDepartment of Neurology. Experimental Research in Stroke and Inflammation (ERSI) Group, University Medical Center Hamburg Eppendorf, Hamburg, Germany.ORCID https://orcid.org/0000-0002-2255-8393
Lucia PaoliniDepartment of Medical and Surgical Specialties, Radiological Sciences and Public Health (DSMC), University of Brescia, Brescia, Italy.ORCID https://orcid.org/0000-0002-4410-5272
Laura J VellaThe Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, Australia.

Funding

Tumor susceptibility gene 101, a new microglial therapy candidate for Alzheimer’s disease to prevent cognitive declineRF1AG082704 · NIA · MAYO CLINIC JACKSONVILLE · PI Seiko Ikezu, Tsuneya Ikezu · 2023 to 2023
$2.0M
Molecular characterization of extracellular vesicles for the spread of misfolded tau proteinR01AG067763 · MAYO CLINIC JACKSONVILLE · 2025 to 2025
$599k
Regulation of bone marrow homing by hematopoietic stem cell vesiclesK08HL175130 · INDIANA UNIVERSITY INDIANAPOLIS · 2025 to 2025
$153k
Alessandra Bono FoundationAnn-Lisa och Bror Björnssons FoundationAssar Gabrielsson Foundation FB23-01Italian Ministry of Health (Ministero della Salute) through the Ricerca Finalizzata (RF) "Theory enhancing" grant RF-2021-12375279Magnus Bergvalls Foundation 2024-1009NHLBI NIH HHS K08 HL175130NIA NIH HHS R01 AG066429NIA NIH HHS R01 AG067763NIA NIH HHS RF1 AG054199NIA NIH HHS RF1 AG082704NIH HHSR01AG066429, R01AG067763, R01AG07271, RF1AG054199, RF1AG082704Serena Ehrenström FoundationVetenskapsrådet Etableringsbidrag (Starting Grant from the Swedish Research Council) 2023-02239Wilhelm och Martina Lundgrens Foundation 2023-SA-4142
6 · The paper itself

Abstract

Solid tissue-derived extracellular vesicles (ST-EVs) are extracellular vesicles (EVs) separated directly from solid tissues of both vertebrates and invertebrates. ST-EVs provide a physiologically relevant snapshot of tissue-specific molecular dynamics and can be enriched directly in situ, from tissues in their natural state, preserving the native characteristics of ST-EVs. However, their enrichment presents unique technical challenges compared to EVs derived from biofluids or cell culture media. The need for transparent reporting in ST-EV research is crucial to enhance the reproducibility, comparability, and reliability of research findings. The Solid Tissue Task Force, part of the Scientific Reproducibility Subcommittee of International Society for Extracellular Vesicles, aims to recommend reporting parameters and identify outstanding questions related to the pre-analytical and analytical handling of solid tissues, as well as ST-EV separation and characterization. These steps are essential for advancing the understanding of the biological roles of ST-EVs and their potential clinical applications.

Indexed as

Extracellular VesiclesAnimalsHumansReproducibility of Resultscharacterizationexosomesextracellular vesiclesguidelinesisolation reproducibilityseparationtissue

Identifiers

PMID41220186
PMCPMC12606037

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.