Evidence map›Paper›PMID 41220334›Full record

ArticleGM crops & food2025

Comparison of two digital PCR platforms for quantification of genetically modified soybean events.

Daniela Verginelli, Sara Ciuffa, Katia Spinella, Davide La Rocca, Daniela Villa, Alessandra Barbante, Elena Perri, Ugo Marchesi

Abstract readComparative Study
In one paragraph

Article in GM crops & food, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Daniela VerginelliNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", Rome, Italy.ORCID 0000-0001-9247-7073
Sara CiuffaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", Rome, Italy.ORCID 0000-0003-1739-3595
Katia SpinellaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", Rome, Italy.ORCID 0000-0003-3839-7974
Davide La RoccaNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", Rome, Italy.ORCID 0000-0001-9456-359X
Daniela VillaCouncil for Agricultural Research and Economics, Research Centre for Plant Protection and Certification - CREA DC, Sede di Tavazzan Tavazzano, Italy.ORCID 0009-0005-0725-8009
Alessandra BarbanteCouncil for Agricultural Research and Economics, Research Centre for Plant Protection and Certification - CREA DC, Sede di Tavazzan Tavazzano, Italy.ORCID 0009-0000-5743-4481
Elena PerriCouncil for Agricultural Research and Economics, Research Centre for Plant Protection and Certification - CREA DC, Sede di Tavazzan Tavazzano, Italy.ORCID 0000-0001-6348-9227
Ugo MarchesiNational Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", Rome, Italy.ORCID 0000-0002-7789-241X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the European Union, the food and feed containing more than 0.9% of approved genetically modified organisms (GMOs) per ingredient must be labeled before placed on the market. In this legislative context, the official control laboratories have to perform validated PCR assays, according to the principles and requirements of ISO/IEC 17025 standard, regarding event-specific methods for the detection, identification and quantification of GMOs. In recent years, with the advent of digital PCR (dPCR) techniques, a growing number of laboratories have transferred the previously validated real-time PCR testings into a dPCR format. Compared to real-time PCR, the dPCR offers the advantage to provide accurate quantification without the need for external calibration samples, show less sensitivity to PCR inhibitors and is more suitable for multiplexing. In this study, an

Indexed as

Glycine maxPlants, Genetically ModifiedPolymerase Chain ReactionReal-Time Polymerase Chain ReactionDigital PCRGMO detectionsoybeanvalidation

Identifiers

PMID41220334
PMCPMC12622311

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.