Evidence map›Paper›PMID 41222238›Full record

ArticlemBio2025

Bacteria export alarmone synthetases that produce (p)ppApp and (p)ppGpp.

Shehryar Ahmad, Andrea G Guedez, Berti Manisa, Abdulmalik Adewale, Kara K Tsang, Vanessa Schiefer, Nathan P Bullen, Harmy Thakar, Youngchang Kim, Boyuan Wang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shehryar Ahmad *Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.ORCID 0000-0002-2747-5212
Andrea G Guedez *Department of Pharmacology, UT Southwestern Medical Center, Dallas, Texas, USA.ORCID 0000-0002-1388-4509
Berti ManisaDepartment of Pharmacology, UT Southwestern Medical Center, Dallas, Texas, USA.
Abdulmalik AdewaleDepartment of Pharmacology, UT Southwestern Medical Center, Dallas, Texas, USA.
Kara K TsangDepartment of Infection Biology, London School of Hygiene and Tropical Medicine, London, England, United Kingdom.
Vanessa SchieferMichael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Nathan P BullenMichael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Harmy ThakarMichael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.
Youngchang KimStructural Biology Center, X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Lemont, Illinois, USA.
Boyuan WangDepartment of Pharmacology, UT Southwestern Medical Center, Dallas, Texas, USA.ORCID 0000-0003-1854-6178
John C WhitneyMichael DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0002-4517-8836

Funding

In-depth characterization of the metabolic effect of the bacterial alamorne ppGppR00GM135536 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI WANG, BOYUAN · 2022 to 2024
$747k
NIGMS NIH HHS R00 GM135536
6 · The paper itself

Abstract

Guanosine penta- and tetraphosphate [(p)ppGpp] and their adenosine analogs [(p)ppApp] are bacterial second messengers known as alarmones. Members of the RelA-SpoT homolog (RSH) family synthesize (p)ppGpp to mediate the stringent response during nutrient starvation, whereas (p)ppApp synthetases have been identified as bactericidal toxins in diverse contexts including type VI secretion systems, toxin-antitoxin modules, and phages. Although alarmone synthesis has traditionally been viewed as a cytoplasmic process, early studies in

Indexed as

Bacterial ProteinsGuanosine PentaphosphateGuanosine TetraphosphateLigasesStreptomycesBacterial ProteinsGuanosine PentaphosphateGuanosine TetraphosphateLigasesalarmonesbacterial protein exportenzyme mechanism(p)ppApp(p)ppGpp

Identifiers

PMID41222238
PMCPMC12691689

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.