Detection of bacteria affected by roasted burdock tea powder and fructooligosaccharide in human faecal culture.

Sae Azuma, Mizuki Sato, Ayaka Nakamura, Hajime Takahashi, Junji Inoue, Shu Takayanagi, Madoka Nakamura, Takashi Kuda

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Article in Molecular biology reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Sae AzumaDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Mizuki SatoDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Ayaka NakamuraDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Hajime TakahashiDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Junji InoueAHJIKAN Co., ltd., 7-3-9, Shoko Center, Nishiku, Hiroshima-city, Hiroshima, 733-8677, Japan.

Shu TakayanagiAHJIKAN Co., ltd., 7-3-9, Shoko Center, Nishiku, Hiroshima-city, Hiroshima, 733-8677, Japan.

Madoka NakamuraDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan.

Takashi KudaDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo, 108-8477, Japan. kuda@kaiyodai.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBurdock root is rich in gut fermentable fructan (inulin). This study aimed to clarify the effects of processed foods containing burdock on the gut microbiota by inoculating faecal slurries into cultures containing roasted burdock tea powder. METHODS AND

resultsHuman faecal slurries (n = 5) were inoculated into cultures containing 4% (w/v) roasted burdock tea powder that washed with 85% (v/v) ethanol (WBT), 0.4% fructooligosaccharide (FO), or both WBT and FO (BF). After 24 h of anaerobic incubation, lactate and acetate levels significantly increased in the BF and WBT cultures. Putrefactive compounds, such as ammonia and indole, were suppressed in BF and WBT cultures. Analysis of 16S rDNA (V3-V4 region) amplicon sequencing revealed that WBT increased the abundance of Bifidobacterium and Faecalibacterium spp. more than twofold; however, the effect varied among individuals. The amplicon sequences were used to predict metabolic functions using Tax4Fun and the Kyoto Encyclopedia of Genes and Genomes database. Histidine biosynthesis was suggested to be promoted in WBT cultures.

conclusionThis in vitro study provides novel insights into the gut microbiota, typical WBT-responsible gut indigenous bacteria, such as Bifidobacterium and Faecalibacterium spp., and fermentable dietary fibre. Overall, these findings show the potential applications of burdock.

Indexed as

ArctiumBacteriaFecesGastrointestinal MicrobiomeOligosaccharidesBifidobacteriumFermentationHumansPowdersRNA, Ribosomal, 16STeafructooligosaccharideOligosaccharidesPowdersRNA, Ribosomal, 16STeaBurdock root tea・Dietary fibre・Human faecal culture・Short-chain fatty acids・Histidine biosynthesis

Identifiers

PMID41222584

DOI10.1016/j.xcrm.2023.101341

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.