ArticleInternational journal of molecular sciences2025
Cardiac Circular RNAs CDR1as, Circ-RCAN2, Circ-C12orf29 Show Cell-Specific Hypoxia-Induced Dysregulation and Distinct In Vitro Effects.
Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- hsa-circKLK3-25 promotes epithelial-mesenchymal transition and malignant progression of prostate cancer through the JNK/ERK signaling pathway.American journal of cancer research · 2026Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular RNAs (circRNAs) are looped RNA molecules with regulatory roles in myocardial infarction and post-infarction cascades. We aimed to (i) confirm the circularity of novel circRNAs (CDR1as, circ-RCAN2, circ-C12orf29) implicated in myocardial infarction, (ii) examine cell-specific regulation patterns under hypoxia, and (iii) assess their effects on cell viability and downstream miRNA targets. Experiments were conducted on porcine cardiac progenitor cells (pCPCs), bone marrow mesenchymal stem cells (pMSCs) and cardiac fibroblasts (pCFs). Circularity was assessed by RNase R treatment, subsequent qPCR, gel electrophoresis and Sanger sequencing. Hypoxia experiments with/without serum deprivation mimicked ischemia. Effects on viability with/without hypoxia (MTT assay) and downstream miRNA targets were assessed via short interfering RNA (siRNA)-mediated knockdown of circ-RCAN2 and circ-C12orf29. Following RNase R treatment, qPCR product electrophoresis demonstrated amplification of singular products for all circRNAs, with backsplice junction amplification confirmed via Sanger sequencing. Serum deprivation and hypoxia resulted in cell-specific circRNA expression patterns, with an upregulation of all candidates in pCPCs across all intervals of hypoxia, an upregulation of circ-RCAN2 and circ-C12orf29 in pMSCs with prolonged hypoxia, and no detectable dysregulation in pCFs. siRNA knockdown of circ-RCAN2 reduced pCF- and increased pMSC-viability. circ-C12orf29 knockdown increased pCPC- and reduced pMSC-viability. circ-C12orf29 knockdown also upregulated ssc-miR-21-5p and ssc-miR-181c in pCPCs, with no detectable targets for circ-RCAN2. In conclusion, CDR1as, circ-RCAN2 and circ-C12orf29 are circular and dysregulated in a time- and cell-type-specific manner following hypoxia. circ-RCAN2 and circ-C12orf29 exhibit cell-type specific effects on viability, with circ-C12orf29 also targeting downstream miRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.