ArticleInternational journal of molecular sciences2025
A Novel Surface Plasmon Resonance Imaging (SPRi) Biosensor for the Determination of Bovine Interleukin-10: Development, Validation, and Application in Biological Fluids.
Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- New SPRi Biosensors for Simultaneous Detection of Tau Protein Isoforms-The Importance of the Ptau181/Total Tau Ratio in Alzheimer's Disease.Biomedicines · 2026Article
- Correlation Between Intrafollicular IL-10, Progesterone, and Bovine Oocyte Developmental Competence.International journal of molecular sciences · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Interleukin-10 (IL-10) is a pleiotropic cytokine that is pivotal in regulating the immune response. Its involvement in the pathophysiology of bovine diseases and its potential influence on oocyte developmental competence make it an important target for diagnostics and research. This study aimed to develop and validate a novel, rapid, and sensitive analytical tool for its quantification. A specific biosensor based on Surface Plasmon Resonance Imaging (SPRi) was developed for the precise quantification of bovine IL-10, utilizing a polyclonal rabbit antibody immobilized on a gold chip for direct capture from complex biological matrices. The method was validated for its analytical performance, including linearity, sensitivity, precision, and selectivity. The developed method is characterized by a wide diagnostic range (1-1000 pg/mL) and high sensitivity, with a limit of detection (LOD) of 0.45 pg/mL and a limit of quantification (LOQ) of 1.49 pg/mL. The biosensor was successfully applied to measure IL-10 concentrations in bovine serum and follicular fluid, revealing significantly higher levels in follicular fluid. The validated SPRi biosensor is a rapid, sensitive, and cost-effective tool for determining IL-10 levels. Its successful application confirms its utility for veterinary diagnostics and highlights its potential for research in reproductive biology, particularly for assessing the follicular microenvironment.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.