Evidence map›Paper›PMID 41230906›Full record

ArticleInvestigative ophthalmology & visual science2025

PRRX1 Orchestrates Pericyte-Myofibroblast Transition in Pathological Retinal Fibrosis.

Zhishang Meng, Dan Liu, Yuqian Hu, Wen Shi, Jianing Qiu, Biying Chen, Yongan Meng, Bin Yan, Youling Liang, Jingkai He and 1 more

Abstract read
In one paragraph

Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhishang MengDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Dan LiuDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Yuqian HuDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Wen ShiDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Jianing QiuDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Biying ChenDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Yongan MengDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Bin YanDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Youling LiangDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Jingkai HeDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.
Jing LuoDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: To investigate the role of PRRX1 in pericyte-myofibroblast transition and its contribution to pathological retinal fibrosis. Methods: Transcriptomic profiling was conducted on human fibrovascular membranes and murine oxygen-induced retinopathy retinas to assess PRRX1 expression and its association with fibrosis-related genes. Single‑cell RNA sequencing was performed on retinal tissues to identify pericyte subpopulations and characterize PRRX1-driven molecular pathways. In vitro, primary mouse retinal pericytes underwent hypoxia with or without small interfering RNA-mediated PRRX1 knockdown to assess fibrotic gene expression and cell migration. In vivo, a laser-induced choroidal neovascularization model was used to assess the effect of PRRX1 silencing on subretinal fibrosis. Results: PRRX1 was significantly upregulated in both human fibrovascular membranes and oxygen-induced retinopathy retinas; single‑cell RNA sequencing revealed its enrichment in pericyte subpopulations undergoing pericyte-myofibroblast transition. PRRX1 knockdown reduced fibrotic gene expression and migratory activity in primary pericytes under hypoxia. In the choroidal neovascularization model, PRRX1 silencing significantly reduced subretinal fibrosis and neovascular lesion area. Conclusions: PRRX1 is a key regulator of pericyte-mediated fibrotic remodeling in the retina. Targeting PRRX1 offers a potential therapeutic approach for retinal fibrosis.

Indexed as

Gene Expression RegulationHomeodomain ProteinsMyofibroblastsPericytesRetinaRetinal DiseasesAnimalsCell MovementCells, CulturedDisease Models, AnimalFibrosisGene Expression ProfilingHumansMaleMiceMice, Inbred C57BLHomeodomain ProteinsPRRX1 protein, human

Identifiers

PMID41230906
PMCPMC12617673

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.