ArticleExperimental & molecular medicine2025
Proteins are a source of glycans found in preparations of glycoRNA.
Article in Experimental & molecular medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Defining cell surface and glycosylated RNAs.Trends in genetics : TIG · 2026Review
- GlycoRNA biology, a naive field with unlimited horizons.Glycoconjugate journal · 2026Article
- Functional regulation of GlycoRNA and progress in malignant tumors.Journal of translational medicine · 2026Review
- Glycosylated RNAs in extracellular vesicles: a new layer of RNA sorting and intercellular communication.Extracellular vesicles and circulating nucleic acids · 2026Article
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Authors and funding
6 authors.
Funding
Abstract
Recent discoveries suggesting that RNA can be modified with sialylated glycans (termed glycoRNA) could broaden our understanding of cellular glycosylation beyond traditional proteins and lipids. However, the pathway of RNA-glycosylation and its biological function remain elusive. Following the original glycoRNA isolation protocol, we also detect labeled glycans in small RNA preparations. However, glycosylated molecules showed resistance to treatment with RNase A/T1 but were sensitive to proteinase K digestion under denaturing conditions. Using liquid chromatography-mass spectrometry-based proteomics we here detect various proteins that copurify with small but not large RNA preparations isolated from human or murine cells, including the glycosylated membrane protein LAMP1. Importantly, we further demonstrate that recombinant soluble LAMP1 can be purified following the glycoRNA isolation method. These findings suggest that glycoproteins copurify with RNA using current glycoRNA purification protocols, thus representing a considerable source of glycans in samples of glycoRNA.
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