Evidence map›Paper›PMID 41233928›Full record

ArticleEuropean journal of medical research2025

LGALS3 promotes liver fibrosis by enhancing the expression and phosphorylation of ERK1/2.

Xin Zheng, Lu Yang, Wenbin Wang, Jingying Sun, Yue Li

Abstract read
In one paragraph

Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. The Role of Galectin-3 in Liver Inflammation and Fibrosis.Journal of inflammation research · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Xin ZhengNO.1, Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang, China.
Lu YangNO.2, Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, No. 64 Zhonghua West Road, Jianhua District, Qiqihar, 161006, Heilongjiang, China.
Wenbin WangNO.1, Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang, China.
Jingying SunDepartment of Brucellosis, Qiqihar Seventh Hospital, Qiqihar, Heilongjiang, China.
Yue LiNO.2, Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, No. 64 Zhonghua West Road, Jianhua District, Qiqihar, 161006, Heilongjiang, China. liyue@qmu.edu.cn.

Funding

Basic Research Operating Funds for Undergraduate Colleges and Universities in Heilongjiang 2024-KYYWF-0363
6 · The paper itself

Abstract

backgroundLiver fibrosis represents the common endpoint of numerous chronic liver diseases. Current therapeutic approaches predominantly focus on alleviating symptoms or addressing the underlying causes, with limited options available for reversing fibrosis. Existing antifibrotic drugs often exhibit low efficacy and carry significant side effects, underscoring the urgent need for novel therapeutic targets. LGALS3, a β-galactoside-binding lectin, participates in inflammation and fibrosis across multiple organs. However, its specific role in liver fibrosis remains poorly understood. This study endeavors to elucidate the cellular and molecular mechanisms by which LGALS3 regulates liver fibrosis and assess its potential as a therapeutic target. SUBJECTS AND

methodsTo clarify the role of LGALS3 in liver fibrosis, a TGF-β1-induced liver fibrosis model was established, and the expression level of LGALS3 was analyzed. LGALS3 overexpression and knockdown cell lines were constructed in LX-2 cell line. RT-qPCR, western blot, and wound healing assay were employed to investigate the impact of LGALS3 on LX-2 cell activation, proliferation, migration, and the ERK1/2 pathway. In the LGALS3 overexpression cell model, PD98059 intervention was applied to mimic the therapeutic effect on liver fibrosis.​

resultsLGALS3 overexpression significantly promoted the proliferation and migration of LX-2 cells, along with the expression and phosphorylation of ERK1/2. Conversely, LGALS3 knockdown and treatment with the PD98059 inhibitor reduced LX-2 cell proliferation and migration, as well as the phosphorylation of ERK1/2.​

conclusionsThis study has clarified the pivotal regulatory role of the LGALS3-ERK1/2 signaling axis in liver fibrosis, It reveals that LGALS3 represents a new and promising molecular mechanism that regulates the phosphorylation of ERK1/2. These findings enhance our understanding of the pathological process of liver fibrosis, suggesting that targeting the LGALS3-ERK1/2 axis may represent a promising target for further investigation in liver fibrosis. It also provides potential targets and innovative directions for the development of anti-liver fibrosis drugs.

Indexed as

Galectin 3Liver CirrhosisMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 3AnimalsBlood ProteinsCell LineCell MovementCell ProliferationGalectinsHumansMaleMiceMitogen-Activated Protein Kinase 1PhosphorylationTransforming Growth Factor beta1Blood ProteinsGalectin 3GalectinsLGALS3 protein, humanMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Transforming Growth Factor beta1ERK1/2LGALS3Liver fibrosisPD98059

Identifiers

PMID41233928
PMCPMC12616999

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.