Evidence mapPaperPMID 41234228Full record

ArticleFrontiers in endocrinology2025

Construction and evaluation of a combined model of NAA/STZ-induced type 2 diabetes with carotid balloon injury in SD rats.

Yanmei Wang, Qian Zhou, Chengshan Li, Xiaojing Chenmou, Chan Zhu, Qiu Chen

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Article in Frontiers in endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Yanmei WangClinical Teaching and Research Department, Ya'an Polytechnic College, Ya'an, Sichuan, China.
Qian ZhouDepartment of Endocrinology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Chengshan LiInternal Medicine Department II, Ya'an Polytechnic College Affiliated Hospital, Ya'an, Sichuan, China.
Xiaojing ChenmouInternal Medicine Department II, Ya'an Polytechnic College Affiliated Hospital, Ya'an, Sichuan, China.
Chan ZhuDepartment of Endocrinology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.
Qiu ChenDepartment of Endocrinology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Diabetic macroangiopathy is a leading cause of disability and mortality in type 2 diabetes mellitus (T2DM) patients, but existing animal models have limitations such as complex genetic manipulation or long induction cycles. This study constructed and evaluated a combined model of nicotinamide (NAA)/streptozotocin (STZ)-induced T2DM and carotid balloon injury in Sprague-Dawley (SD) rats. Methods: Sixty male SD rats were divided into four groups: control, VBI (only vascular balloon injury), T2DM (only NAA/STZ-induced T2DM), and T2DM+VBI (combined model) groups. T2DM was induced via NAA (110 mg/kg) followed by STZ (65 mg/kg); VBI was performed via a percutaneous transluminal coronary angioplasty (PTCA) balloon catheter. Over 6 weeks, metabolic indicators [intake, excretion, body weight, and random blood glucose (RBG)] were monitored; carotid tissues were collected at 2, 4, and 6 weeks post-VBI for HE staining and immunohistochemistry (α-SMA, F4/80, and MMP-9); and blood cell counts and plasma biochemistry were analyzed. Results: The T2DM induction success rate was 90% (no mortality); the postoperative mortality rate was 14.3% in the T2DM+VBI group (vs. 7% in the VBI group). The T2DM and T2DM+VBI groups presented typical diabetic symptoms and persistent hyperglycemia. Histologically, only the VBI and T2DM+VBI groups exhibited time-dependent neointimal proliferation, with T2DM+VBI exhibiting a larger neointimal area, a greater proliferation index, more severe luminal stenosis (2-week hyperplasia comparable to VBI 6 weeks) and increased F4/80-positive macrophages/MMP-9 (poor plaque stability). No major organ abnormalities (except T2DM hepatic steatosis) or intergroup blood parameter differences were found. Conclusion: This combined model has stable hyperglycemia, obvious neointimal hyperplasia, rapid modeling and a high success rate (optimal observation window: 2-4 weeks post-VBI). It recapitulates diabetic macroangiopathy pathology and is suitable for investigating therapeutic efficacy and related molecular/cellular mechanisms.

Indexed as

Carotid Artery InjuriesDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2Disease Models, AnimalNiacinamideAnimalsBlood GlucoseMaleRatsRats, Sprague-DawleyStreptozocinBlood GlucoseNiacinamideStreptozocindiabetic macroangiopathyneointimal hyperplasianicotinamide (NAA)streptozotocin (STZ)type 2 diabetes mellitus (T2DM)vascular balloon injury (VBI)

Identifiers

PMID41234228
PMCPMC12605494

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.