Evidence map›Paper›PMID 41236552›Full record

ArticlePediatric surgery international2025

Identification and validation of oxidative stress-related genes in biliary atresia.

Liying Rong, Mengxin Zhang, Junni Ma, Xiaoyu Wang, Jiayu Zheng, Xi Zhang, Shao-Tao Tang

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Article in Pediatric surgery international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Liying RongDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Mengxin ZhangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Junni MaDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xiaoyu WangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Jiayu ZhengDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Xi ZhangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Shao-Tao TangDepartment of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. tshaotao83@hust.edu.cn.

Funding

National Natural Science Foundation of China 82371718
6 · The paper itself

Abstract

purposeIncreasing evidence has indicated a role of oxidative stress in the pathogenesis of biliary atresia (BA). This study aimed to identify key oxidative stress-related biomarkers in BA and explore their therapeutic potential.

methodsBA datasets were obtained from the GEO database. Differentially expressed genes (DEGs) were identified using the GSE46960 dataset. These DEGs were intersected with oxidative stress-related genes from GeneCards to obtain the oxidative stress-related DEGs (ORDEGs). Functional enrichment (GO, KEGG) and gene set enrichment analyses were performed. Hub genes were identified using machine learning and protein-protein interaction (PPI) network analysis. Their expression was validated via qRT-PCR in BA patient liver samples and rhesus rotavirus (RRV)-induced mouse models. Potential transcription factors (TFs), microRNAs, and drugs targeting hub ORDEGs were predicted using TRRUST, TarBase, and DSigDB, respectively.

resultsSix hub ORDEGs (CCL2, CXCL8, SPP1, EDN1, TGFB3, and F3) were ultimately identified. Their mRNA levels were significantly upregulated in both BA patients and mice. A total of 52 TFs and 13 microRNAs were identified. Among the predicted drugs, simvastatin may hold therapeutic potential.

conclusionThis study identified six key ORDEGs (CCL2, CXCL8, SPP1, EDN1, TGFB3, and F3) to be important pathogenic factors and potential therapeutic targets in BA.

Indexed as

Biliary AtresiaOxidative StressAnimalsBiomarkersDisease Models, AnimalGene Expression ProfilingHumansMiceMicroRNAsBiomarkersMicroRNAsBiliary atresiaBioinformatic analysisBiomarkerMachine learningOxidative stress

Identifiers

PMID41236552

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.