ArticleFolia microbiologica2025
Blue-green pigment production by environmental isolates of Pseudomonas aeruginosa: Purification and bioactivity.
Article in Folia microbiologica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A total of 65 blue-green pigment-producing bacterial strains were isolated from different environmental sources. The strains were identified as Pseudomonas aeruginosa Z57 and Pseudomonas aeruginosa Z62 using the Vitek2 identification system. Both strains were confirmed to be Gram-negative and exhibited positive reactions for several enzymes, including glutamyl arylamidase, d-glucose, gamma-glutamyl-transferase, d-mannose, beta-alanine arylamidase, and proline arylamidase, while testing negative for others. Optimal growth conditions were established at 35°C and pH 7, utilizing glucose and casein as carbon and nitrogen sources, respectively. The most potent isolate, Pseudomonas aeruginosa WM1, was confirmed by 16S rRNA sequencing and registered in Gene Bank with Accession No. (PV055704). Pyocyanin was extracted, and then purified using column chromatography. It was further characterized by UV-Vis Spectrophotometer, Fourier transformer Infrared spectrometry (FTIR), and nuclear magnetic resonance (NMR). Antimicrobial assays showed that higher concentrations of purified pyocyanin resulted in larger inhibition zones against pathogenic microbes. Interestingly, bacterial strains demonstrated greater resistance to pyocyanin than fungal strains; at a concentration of 2.5 µg/mL, Staphylococcus aureus and Aspergillus niger were identified as the most resistant organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.