Evidence mapPaperPMID 41239833Full record

ArticleEuropean journal of clinical investigation2026

Assessing the interaction of the UCP system and fatty acids on epicardial adipose tissue mitochondrial respiration.

Diana Santos, Ana Burgeiro, Ana Catarina R G Fonseca, Cândida Dias, Teresa Cunha-Oliveira, Aryane Oliveira, João Laranjinha, António Canotilho, Gonçalo Coutinho, David Prieto and 3 more

Abstract read
In one paragraph

Article in European journal of clinical investigation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Diana SantosPhD Programme in Experimental Biology and Biomedicine (PDBEB), University of Coimbra, Institute for Interdisciplinary Research, Coimbra, Portugal.ORCID https://orcid.org/0000-0002-9292-5961
Ana BurgeiroCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Ana Catarina R G FonsecaCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Cândida DiasCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Teresa Cunha-OliveiraCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.ORCID https://orcid.org/0000-0002-7382-0339
Aryane OliveiraCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
João LaranjinhaCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
António CanotilhoCardiothoracic Surgery Unit at the University Hospital of Coimbra, Coimbra, Portugal.
Gonçalo CoutinhoCardiothoracic Surgery Unit at the University Hospital of Coimbra, Coimbra, Portugal.
David PrietoCardiothoracic Surgery Unit at the University Hospital of Coimbra, Coimbra, Portugal.
Pedro AntunesCardiothoracic Surgery Unit at the University Hospital of Coimbra, Coimbra, Portugal.
Manuel AntunesUniversity Clinic for Cardiothoracic Surgery, Faculty of Medicine, University Hospital of Coimbra, Coimbra, Portugal.
Eugenia CarvalhoCNC-UC - Centre for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.

Funding

European Regional Development Fund CENTRO-01-0145-FEDER-000012European Regional Development Fund COMPETE 2020Fundação para a Ciência e a Tecnologia LA/P/0058/2020Fundação para a Ciência e a Tecnologia POCI-01-0145-FEDER-007440Fundação para a Ciência e a Tecnologia SFRH/BD/144199/2019Fundação para a Ciência e a Tecnologia SFRH/PBD/101030/2014Fundação para a Ciência e a Tecnologia UIDB/04539/2020Fundação para a Ciência e a Tecnologia UIDI/04539/2020
6 · The paper itself

Abstract

backgroundEpicardial adipose tissue (EAT) exhibits brown-like features, including the expression of uncoupling protein 1 (UCP1). EAT interacts dynamically with cardiac cells to modulate local cardiac tissue physiology and metabolic function. No studies have evaluated the impact of UCP1 inhibition on oxidative phosphorylation (OXPHOS) in fresh EAT explants. This study aimed to determine the unique bioenergetic characteristics of fresh EAT explants by comparing it to subcutaneous adipose tissue (SAT). Furthermore, the key impact of UCP1 inhibition on EAT respiration and how this process is influenced by the presence of type 2 diabetes mellitus (DM) or coronary artery disease (CAD), was also evaluated.

methodsEAT and SAT biopsies were collected from 205 (151 male and 51 female) study participants, undergoing cardiac surgery. Participants were stratified according to the presence/absence of DM or CAD. Markers of mitochondrial content and bioenergetics were evaluated.

resultsEAT demonstrated a higher bioenergetic activity compared to SAT, in both nicotinamide adenine dinucleotide (NADH)-linked and fatty acid oxidation (FAO)-linked OXPHOS. Importantly, UCP1 inhibition with guanosine 5'-diphosphate (GDP), flattens the differences between the tissues in the NADH-linked OXPHOS; in contrast these differences were potentiated in the FAO-linked OXPHOS. Minor differences in mitochondrial content and respiration were observed when subjects were stratified according to either DM or CAD.

conclusionsThis study emphasizes the important bioenergetic differences between EAT and SAT, which are crucial in the context of the local cardiomyocyte metabolism, as well as the impact of UCP1 inhibition in EAT. A deeper understanding of the unique characteristics of EAT and its metabolic micro-environment may provide valuable insights into the cardiovascular disease pathologies.

Indexed as

Adipose TissueFatty AcidsMitochondriaPericardiumSubcutaneous FatUncoupling Protein 1AgedCell RespirationCoronary Artery DiseaseDiabetes Mellitus, Type 2Energy MetabolismEpicardial Adipose TissueFemaleHumansMaleMiddle AgedFatty AcidsUCP1 protein, humanUncoupling Protein 1cardiovascular diseaseepicardial adipose tissuefatty acid oxidationmitochondrial respirationuncoupling protein 1

Identifiers

PMID41239833
PMCPMC12820919

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.