ArticleRespiratory research2025
Regulation of T cell tissue residency and activation in human PCLS.
Article in Respiratory research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Preparation and Infection of Bovine Precision-Cut Lung Slices (b-PCLS) as an Ex Vivo Model for Respiratory Pathogens.Current protocols · 2026Article
- Article
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15 authors.
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Abstract
backgroundResident immune cells are central in shaping the lung's tissue-specific immunity. Precision-cut lung slices (PCLS) preserve the native tissue microenvironment and are therefore an excellent ex vivo model to analyze residency and functionality of resident memory T cells.
methodsTo study the modulation of tissue residency markers and T cell activation in the native lung niche, we treated PCLS with broad and T cell-specific stimuli and analyzed responses using flow cytometry and mediator secretion analysis. Using TGFβ, anti-CD3/CD28, IL-2 and a pool of MHC-I restricted peptides we analyzed cytokine secretion, CD4
resultsFirst, we characterized lung immune cell in PCLS which also revealed that resident memory T cells are abundant in PCLS. We showed that regulation of the tissue residency marker CD103 is dependent on TGFβ or IL-2 signaling in combination with T cell receptor engagement. Further, polyclonal activation of T cells in the tissue reduced tissue secretion of anti-inflammatory cytokines like TGFβ, while increasing the secretion of T cell-associated cytokines like IFNγ, IL-2, and Granzyme B. This shift was supported by an upregulation of T cell activation markers such as CD39, CD137, and Ki-67. Finally, treatment of PCLS with a pool of MHC-I-restricted peptides led to increased secretion of multiple inflammatory effector cytokines associated and a specific activation of tissue resident T cells.
conclusionTaken together, we have demonstrated that PCLS provide an excellent platform to modulate tissue resident T cell responses influenced by human lung tissue microenvironment.
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