ArticleMethods in molecular biology (Clifton, N.J.)2026
Identification of Potentially Coding Small ORFs in Plant Transcriptomes.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Small open reading frames (smORFs, <100 amino acids) are a ubiquitous feature of eukaryotic and prokaryotic genomes. However, only a fraction of these smORFs encode functional proteins, known as microproteins. Functional microproteins have been found in all three domains of life, although due to limitations in their prediction and characterization, only a few have been described in plants. Therefore, the catalogization and functional analysis of plant smORF-encoded microproteins is far from complete. In this chapter, we present a bioinformatic pipeline designed to predict and validate coding smORFs in plant transcriptomes. The workflow comprises several key steps: transcriptome assembly, prediction of potentially coding smORFs, filtration of candidates, and validation of their translation through analysis of mass-spectrometry data.
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