Evidence map›Paper›PMID 41243428›Full record

ArticleZoonoses and public health2026

Serosurveillance of Leishmania infantum in Zoo-Kept Animals in Spain.

Jesús Barbero-Moyano, Diana Marteles, David Cano-Terriza, Sergio Villanueva-Saz, Moisés Gonzálvez, Pablo Quilez, Adrián Beato-Benítez, Javier Martínez, Alfredo Gargallo-Martín, Pilar Soriano and 2 more

Abstract read
In one paragraph

Article in Zoonoses and public health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jesús Barbero-MoyanoDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba, Spain.
Diana MartelesDepartamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain.
David Cano-TerrizaDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba, Spain.ORCID https://orcid.org/0000-0001-5657-2567
Sergio Villanueva-SazDepartamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain.ORCID https://orcid.org/0000-0001-6209-4282
Moisés GonzálvezDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba, Spain.ORCID https://orcid.org/0000-0003-0423-3189
Pablo QuilezDepartamento de Patología Animal, Universidad de Zaragoza, Zaragoza, Spain.ORCID https://orcid.org/0009-0003-9757-3840
Adrián Beato-BenítezDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba, Spain.
Javier MartínezBioparc Valencia, Valencia, Spain.
Alfredo Gargallo-MartínSelwo Aventura, Estepona, Málaga, Spain.
Pilar SorianoRío Safari Elche, Alicante, Spain.
María A RisaldeCIBERINFEC, ISCIII - CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III, Madrid, Spain.
Ignacio García-BocanegraDepartamento de Sanidad Animal, Grupo de Investigación en Sanidad Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, Córdoba, Spain.ORCID https://orcid.org/0000-0003-3388-2604

Funding

CIBER-Consorcio Centro de Investigación Biomédica en Red CB 2021Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Aragón Government AR15_23RUnión Europea-Next Generation EU
6 · The paper itself

Abstract

introductionLeishmaniosis is a sand fly-borne zoonosis mainly caused by Leishmania infantum in Europe. Exposure to this protozoan has been widely reported in many domestic and wild species. However, epidemiological surveys evaluating the circulation of L. infantum in zoo-kept animals remain limited. This large-scale study aims to evaluate the seroprevalence of L. infantum in zoo-kept species in Spain as well as alterations in serum protein levels in L. infantum-seropositive individuals, to identify potential risk factors associated with L. infantum exposure, and to assess the dynamics of seropositivity in animals longitudinally sampled during the study period.

methodsBetween 2007 and 2023, serum samples from 429 zoo-kept animals belonging to 72 species were collected in nine zoos in Spain using convenience sampling. Additionally, 29 of these individuals from six of the tested zoos were also longitudinally sampled.

resultsAnti-L. infantum antibodies were detected in 22 (5.1%; 95% CI: 3.0-7.2) of the 429 animals using an in-house ELISA, as well as in 13.9% (10/72) and 66.7% (6/9) of the species and zoos tested, respectively. Serum protein electrophoresis analyses revealed that polyclonal gammopathy was the most common alteration in L. infantum-seropositive individuals. Three animals longitudinally surveyed seroconverted throughout the study period. The multivariate analysis identified the family Canidae as a risk factor for L. infantum exposure.

conclusionsOur results indicate a moderate, widespread and endemic circulation of L. infantum in zoo-kept animals from Spain, which may be of animal health, conservation, and public health concern. Surveillance programs and control measures should be implemented in zoos to minimise the exposure of these species to Leishmania spp., particularly in hotspot areas.

Indexed as

Animals, ZooLeishmania infantumLeishmaniasis, VisceralAnimalsAntibodies, ProtozoanFemaleMaleRisk FactorsSeroepidemiologic StudiesSpainAntibodies, ProtozoanELISALeishmania infantumserum proteinvector‐bornezoozoonoses

Identifiers

PMID41243428
PMCPMC12765971

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.