Evidence map›Paper›PMID 41246403›Full record

ArticleOpen veterinary journal2025

Cysteine reduces oxidative damage induced by heat stress in dromedary camel oocytes during

Nasser Ghanem, Mohamed Eleam, Gamal Shawki, Marwa El-Sheikh, Omar A Farid, Beshoy S F Khalil, Fatma Badawy Aboelwafa, Najmi Mariol, Ayman Mesalam

Abstract read
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In one paragraph

Article in Open veterinary journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nasser GhanemAnimal Production Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
Mohamed EleamDepartment of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Gamal ShawkiDepartment of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Marwa El-SheikhDepartment of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre (NRC), Dokki, Cairo, Egypt.
Omar A FaridNational Organization for Drug Control and Research, Giza, Egypt.
Beshoy S F KhalilGenetics Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
Fatma Badawy AboelwafaGenetics Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
Najmi MariolDepartment of Surgery and Theriogenology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Ayman MesalamDepartment of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Assisted reproductive technologies (ARTs), including Aim: This study aimed to evaluate the potential effects of cysteine supplementation in IVM medium on nuclear maturation, mitochondrial activity, antioxidant status, oocyte ultrastructure, and heat stress marker genes expression using a dromedary camel oocyte model. Methods: Two experiments were conducted. In Experiment 1, cumulus-oocyte complexes (COCs) were collected and matured Results: Results showed that heat stress conditions significantly reduced nuclear maturation, mitochondrial activity, ATP and antioxidant levels, and the mRNA expression levels of antioxidant, anti-apoptotic, cumulus expansion, and oocyte quality markers, while increasing pro-apoptotic Conclusion: Supplementation with 1.6 mM cysteine during IVM of camel oocytes effectively improves oocyte quality and mitigates heat stress-related damage. This research supports the practical application of cysteine as a supplement to IVM media, specifically to enhance oocyte developmental competence and improve ART success rates in camels exposed to thermal stress.

Indexed as

CamelusCysteineHeat-Shock ResponseIn Vitro Oocyte Maturation TechniquesOocytesOxidative StressAnimalsAntioxidantsFemaleHot TemperatureAntioxidantsCysteineAntioxidant activityCysteineDromedary camelHeat stressIVM

Identifiers

PMID41246403
PMCPMC12613221

What Socratic holds

Textmetadata
LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.