Evidence map›Paper›PMID 41249569›Full record

ArticleArchives of microbiology2025

Antibacterial and antibiofilm efficacy of ar-turmerone derived from Curcuma longa L. arrested multidrug-resistant pathogenic Gram-positive bacteria.

Neha Panwar, Rambir Singh, Satendra Singh, Manisha Tiwari, Pratibha Mehta Luthra

Abstract read
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In one paragraph

Article in Archives of microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Neha PanwarNeuropharmaceutical Chemistry Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, North Campus, University of Delhi, Delhi, 110007, India.
Rambir SinghNeuropharmaceutical Chemistry Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, North Campus, University of Delhi, Delhi, 110007, India.
Satendra SinghNeuropharmaceutical Chemistry Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, North Campus, University of Delhi, Delhi, 110007, India.
Manisha TiwariNeuropharmaceutical Chemistry Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, North Campus, University of Delhi, Delhi, 110007, India.
Pratibha Mehta LuthraNeuropharmaceutical Chemistry Laboratory, Dr. B. R. Ambedkar Center for Biomedical Research, North Campus, University of Delhi, Delhi, 110007, India. pmluthra@acbr.du.ac.in.

Funding

Institution of Eminence (IoE) IoE/2023-24/12/FRP
6 · The paper itself

Abstract

The antibacterial and antibiofilm efficacy of ar-turmerone, a major constituent of Curcuma longa essential oil, against Gram-positive clinical pathogenic strains of bacteria was evaluated in this study. ar-turmerone was extracted from turmeric essential oil using silica gel column chromatography and characterized by nuclear magnetic resonance (NMR), liquid chromatography-mass spectrometry (LC-MS), and high-performance liquid chromatography (HPLC). Antibacterial efficacy was tested via broth microdilution to determine minimum inhibitory concentration (MIC) values against Staphylococcus aureus (ATCC and clinical isolate), S. epidermidis clinical isolate, Enterococcus faecalis clinical isolate, and Streptococcus viridans clinical isolate. Biofilm inhibition (minimum biofilm inhibitory concentration, MBIC) and eradication (minimum biofilm eradication concentration, MBEC) were assessed using crystal violet assays and bactericidal effect was then confirmed using resazurin viability assay and live/dead fluorescence microscopy. Ar-turmerone's activity was compared with its crude source, turmeric essential oil, to demonstrate the potency gained upon purification. Gentamicin and quercetin served as controls for antibacterial and antibiofilm effects, respectively. Scanning electron microscopy confirmed disruption of biofilm structure post-treatment. Ar-turmerone exhibited significantly lower MIC, MBIC and MBEC values than the turmeric essential oil and the standard drugs. Cytotoxicity testing on HEK 293 and L929 fibroblast cells showed that ar-turmerone was highly biocompatible (IC₅₀ = 11.09 mM in HEK 293; no cytotoxicity in L929 up to 9.25 mM). This study provides a comprehensive in vitro evaluation of ar-turmerone's antibacterial, biofilm inhibitory and eradication potential. The efficacy of ar-turmerone against biofilm-forming, drug-resistant Gram-positive pathogens and low cytotoxicity established the compound for future development as a plant-derived antimicrobial agent.

Indexed as

Anti-Bacterial AgentsBiofilmsCurcumaDrug Resistance, Multiple, BacterialGram-Positive BacteriaPlant ExtractsHumansMicrobial Sensitivity TestsOils, VolatileStaphylococcus aureusAnti-Bacterial AgentsOils, VolatilePlant ExtractsAntimicrobial activityar-turmeroneBiofilmsCurcuma longa rhizomeCytotoxicityHEK 293 cellsMultidrug resistancePathogenic bacteriaSEM imaging

Identifiers

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.